Anti-Centrobin 抗体 (ab70448)
Key features and details
- Mouse polyclonal to Centrobin
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Centrobin antibody -
製品の詳細
Mouse polyclonal to Centrobin -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein within Human Centrobin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (AAH21134.1, 1 a.a. ~ 903 a.a).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70448の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/1000. Detects a band of approximately 135 kDa (predicted molecular weight: 102 kDa).
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特記事項 |
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WB
1/500 - 1/1000. Detects a band of approximately 135 kDa (predicted molecular weight: 102 kDa). |
ターゲット情報
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機能
Required for centriole duplication. Inhibition of centriole duplication leading to defects in cytokinesis. -
組織特異性
Widely expressed (at protein level). Highly expressed in testis. Also expressed in spleen, thymus, prostate, small intestine, colon and peripheral blood leukocytes. -
発生段階
Preferentially incorporated into the newly assembled daughter centriole during centriole assembly at the late G1 or early S phase. Remains in the daughter centrioles throughout the cell cycle. At the next cycle of centriole duplication, its amount on the original daughter centriole eventually decreases. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Cytoplasm > cytoskeleton > centrosome > centriole. Centriole-associated, asymmetrically localizes to the daughter centriole. - Information by UniProt
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参照データベース
- Entrez Gene: 116840 Human
- Omim: 611425 Human
- SwissProt: Q8N137 Human
- Unigene: 348012 Human
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別名
- 9830165K03Rik antibody
- Centrobin antibody
- centrobin, centrosomal BRCA2 interacting protein antibody
see all
画像
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All lanes : Anti-Centrobin antibody (ab70448) at 1/500 dilution
Lane 1 : Centrobin transfected 293T lysate
Lane 2 : Non-transfected 293T lysate
Lysates/proteins at 25 µg per lane.
Secondary
Lane 1 : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Lane 2 : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size: 102 kDa
Observed band size: 135 kDa why is the actual band size different from the predicted?
データシートおよび資料
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Datasheet download
参考文献 (22)
ab70448 は 22 報の論文で使用されています。
- Moraes de Farias K et al. The Isoflavanoid (+)-PTC Regulates Cell-Cycle Progression and Mitotic Spindle Assembly in a Prostate Cancer Cell Line. Chem Biodivers 19:e202200102 (2022). PubMed: 35362194
- Balestra FR et al. TRIM37 prevents formation of centriolar protein assemblies by regulating Centrobin. Elife 10:N/A (2021). PubMed: 33491649
- Kong D & Loncarek J Analyzing Centrioles and Cilia by Expansion Microscopy. Methods Mol Biol 2329:249-263 (2021). PubMed: 34085228
- Chen TY et al. Septin 7 is a centrosomal protein that ensures S phase entry and microtubule nucleation by maintaining the abundance of p150glued. J Cell Physiol 236:2706-2724 (2021). PubMed: 32869310
- Meitinger F et al. TRIM37 prevents formation of condensate-organized ectopic spindle poles to ensure mitotic fidelity. J Cell Biol 220:N/A (2021). PubMed: 33983387