製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to CNTNAP3
  • アプリケーション
    適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide from a region within internal amino acids 612-661 SGPLGPFLVY CNMTADAAWT VVQHGGPDAV TLRGAPSGHP RSAVSFAYAA, of Human CNTNAP3 (NP_387504).

  • ポジティブ・コントロール
    • DU145 cell lysate This antibody gave a positive result when used in the following formaldehyde fixed cell lines: DU145

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab89720 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 141 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 配列類似性
    Belongs to the neurexin family.
    Contains 2 EGF-like domains.
    Contains 1 F5/8 type C domain.
    Contains 1 fibrinogen C-terminal domain.
    Contains 4 laminin G-like domains.
  • 細胞内局在
    Secreted and Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CASPR3 antibody
    • Cell recognition molecule Caspr3 antibody
    • CNTNAP3 antibody
    • CNTNAP3A antibody
    • CNTP3_HUMAN antibody
    • Contactin associated protein like 3 antibody
    • Contactin-associated protein-like 3 antibody
    • FLJ14195 antibody
    • KIAA1714 antibody
    see all

画像

  • ICC/IF image of ab89720 stained DU145 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab89720 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-CNTNAP3 antibody (ab89720) at 1 µg/ml (in 5% skim milk / PBS buffer) + DU145 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 141 kDa
    Observed band size : ~144 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.

参考文献

ab89720 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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