製品の概要

  • 製品名
    Anti-CNTF Receptor alpha antibody
    CNTF Receptor alpha 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to CNTF Receptor alpha
  • 由来種
    Goat
  • 特異性
    This antibody is expected to recognise both reported isoforms (NP_671693.1; NP_001833.1).
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Chicken, Dog, Pufferfish, Zebrafish
  • 免疫原

    Synthetic peptide:

    CRSNTYPKGFY

    , corresponding to internal sequence amino acids 116-126 of Human CNTF Receptor alpha

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • ポジティブ･コントロール
    • Mouse Brain lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab58560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).

ターゲット情報

  • 機能
    Binds to CNTF. The alpha subunit provides the receptor specificity.
  • 組織特異性
    Nervous system and skeletal muscle.
  • 配列類似性
    Belongs to the type I cytokine receptor family. Type 3 subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
  • ドメイン
    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
    see all
  • 別名
    • Ciliary neurotrophic factor receptor alpha antibody
    • Ciliary neurotrophic factor receptor antibody
    • Ciliary neurotrophic factor receptor subunit alpha antibody
    • CNTF receptor subunit alpha antibody
    • CNTFR Alpha antibody
    • CNTFR antibody
    • CNTFR-alpha antibody
    • CNTFR_HUMAN antibody
    see all

画像

  • Western blot - Anti-CNTF Receptor alpha antibody (ab58560)
    Western blot - Anti-CNTF Receptor alpha antibody (ab58560)
    Anti-CNTF Receptor alpha antibody (ab58560) at 0.3 µg/ml + Mouse Brain lysate (35µg protein in RIPA buffer)

    Predicted band size: 41 kDa
    Observed band size: 41 kDa



    Primary incubation was 1 hour. Detected by chemiluminescence.
  • Western blot - Anti-CNTF Receptor alpha antibody (ab58560)
    Western blot - Anti-CNTF Receptor alpha antibody (ab58560)
    All lanes : Anti-CNTF Receptor alpha antibody (ab58560) at 1 µg/ml

    Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330) at 20 µg
    Lane 2 : Mouse skeletal muscle tissue lysate - total protein (ab29711) at 20 µg
    Lane 3 : Skeletal Muscle (Rat) Tissue Lysate - normal tissue (ab29376) at 20 µg
    Lane 4 : Human brain tissue lysate - total protein (ab29466) at 10 µg
    Lane 5 : Brain (Mouse) Tissue Lysate (ab27253) at 10 µg
    Lane 6 : Brain (Rat) Tissue Lysate (ab7942) at 10 µg

    Secondary
    All lanes : Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution

    Predicted band size: 41 kDa

プロトコール

参考文献

This product has been referenced in:
  • Li R  et al. CNTF Mediates Neurotrophic Factor Secretion and Fluid Absorption in Human Retinal Pigment Epithelium. PLoS One 6:e23148 (2011). WB ; Human . Read more (PubMed: 21912637) »

See 1 Publication for this product

ab58560 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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