Anti-CNOT2 抗体 (ab55679)

製品の概要

  • 製品名Anti-CNOT2 antibody
    CNOT2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to CNOT2
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Recombinant fragment: EDLLFYLYYM NGGDVLQLLA AVELFNRDWR YHKEERVWIT RAPGMEPTMK TNTYERGTYY FFDCLNWRKV AKEFHLEYDK LEERPHLPST FNYNPAQQAF , corresponding to amino acids 441-541 of Human CNOT2

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノモノクローナル
  • アイソタイプIgG2a
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab55679 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 60 kDa.
ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 機能The CCR4-NOT complex functions as general transcription regulation complex.
  • 組織特異性Ubiquitous. Highly expressed in brain, heart, thymus, spleen, kidney, liver, small intestine, placenta, lung and peripheral blood leukocytes.
  • 配列類似性Belongs to the CNOT2/3/5 family.
  • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Cytoplasm. Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CCR4 associated factor 2 antibody
    • CCR4 NOT transcription complex subunit 2 antibody
    • CCR4-associated factor 2 antibody
    • CCR4-NOT transcription complex subunit 2 antibody
    • CDC36 antibody
    • CNOT2 antibody
    • CNOT2_HUMAN antibody
    • HSPC131 antibody
    • MSTP046 antibody
    • Negative regulator of transcription 2 antibody
    • NOT2 (negative regulator of transcription 2 yeast) homolog antibody
    • NOT2 antibody
    • NOT2H antibody
    see all

Anti-CNOT2 antibody 画像



  • Predicted band size : 60 kDa
    CNOT2 antibody (ab55679) at 1ug/lane + A-549 cell lysate at 25ug/lane.
  • ab55679 staining CNOT2 in mouse ES cells by Immunocytochemistry/ Immunofluorescence. The cells were methanol fixed and then blocked using 0.2% fish scale gelatin for 1 hour at 25°C. Samples were then incubated with primary antibody at 1/300 for 20 minutes at 25°C. The secondary antibody used was a donkey anti-mouse IgG conjugated to Alexa Fluor® 594 (red) used at a 1/500 dilution. DAPI was used to stain the cell nuclei (blue).

    See Abreview

Anti-CNOT2 antibody (ab55679) 使用論文

ab55679 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Loading amount 30 µg
Specification HeLa
Gel Running Conditions Reduced Denaturing (11)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

投稿 Feb 16 2012

Vielen Dank für Ihre Email. Es tut mir leid zu hören, dass Sie Probleme mit diesem Antikörper haben. Wir haben zur Zeit kein anders Feedback bekommen, dass es ein Problem hätte mit diesem Antikörper. Wir nehmen jedoch alle Produktbeschwerden se...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (mouse ES cells)
Specification mouse ES cells
Fixative Methanol
Permeabilization No
Blocking step Fish scale gelatin as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Oct 06 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"