製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to CNBP
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Guinea pig, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (SSNECFKCGR SGHWARECPT GGGRGRGMRS RGRGGFTSDR GFQFVSSSLP) of Human CNBP (NP_003409).

  • ポジティブ・コントロール
    • Human fetal brain lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab83038 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1.777777778.

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 19 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    Single stranded DNA-binding protein, with specificity to the sterol regulatory element (SRE). Involved in sterol-mediated repression.
  • 組織特異性
    Present in all tissues examined.
  • 関連疾患
    Defects in CNBP are the cause of dystrophia myotonica type 2 (DM2) [MIM:602668]; also known as proximal myotonic myopathy (PROMM). A multisystem disease characterized by the association of proximal muscle weakness with myotonia, cardiac manifestations and cataract. Additional features can include hyperhidrosis, testicular atrophy, insulin resistance and diabetes and central nervous system anomalies in rare cases. Note=The causative mutation is a CCTG expansion (mean approximately 5000 repeats) located in intron 1 of the CNBP gene.
  • 配列類似性
    Contains 7 CCHC-type zinc fingers.
  • 細胞内局在
    Cytoplasm. Endoplasmic reticulum.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CCHC type zinc finger nucleic acid binding protein antibody
    • Cellular nucleic acid binding protein antibody
    • Cellular nucleic acid-binding protein antibody
    • CNBP antibody
    • CNBP_HUMAN antibody
    • CNBP1 antibody
    • DM2 antibody
    • Erythroid differentiation related antibody
    • PROMM antibody
    • Proximal myotonic myopathy nucleic acid binding protein antibody
    • RNF163 antibody
    • Sterol regulatory element binding protein antibody
    • ZCCHC22 antibody
    • Zinc finger protein 273 antibody
    • Zinc finger protein 9 (a cellular retroviral nucleic acid binding protein) antibody
    • Zinc finger protein 9 antibody
    • ZNF9 antibody
    see all

Anti-CNBP antibody 画像

  • Anti-CNBP antibody (ab83038) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 19 kDa
    Observed band size : 21 kDa (why is the actual band size different from the predicted?)

プロトコール

Anti-CNBP antibody (ab83038) 使用論文

This product has been referenced in:
  • Lee E  et al. CNBP acts as a key transcriptional regulator of sustained expression of interleukin-6. Nucleic Acids Res 45:3280-3296 (2017). Read more (PubMed: 28168305) »

See 1 Publication for this product

Product Wall

Application
Western blot
Sample
Mouse Cell lysate - whole cell (bone marrow derived macrophages)
Gel Running Conditions
Reduced Denaturing (13%)
Loading amount
500000 cells
Specification
bone marrow derived macrophages
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Jan 18 2016

I am very pleased to hear you would like to accept our offer and test ab83038 in ICC/IF. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ICC/IF and include this code in...

Read More

Thank you very much for your interest in our anti-CNBP antibodies. To our knowledge, neither ab48027 nor ab83038 have been tested in cell staining (immunocytochemistry, ICC/IF). The information about localization in the ER and cytoplasm comes f...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録