Anti-CNAP1 抗体 (ab34338)

製品の概要

  • 製品名Anti-CNAP1 antibody
    CNAP1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CNAP1
  • アプリケーション適用あり: IHC-P, WBmore details
    適用なし: ICC/IF
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide derived from within residues 1350 to the C-terminus of Human CNAP1.

    (Peptide available as ab34337.)

  • ポジティブ・コントロール
    • HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate, Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate, A431 (Human epithelial carcinoma cell line) Whole Cell Lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab34338 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 157 kDa).
  • 追加情報Is unsuitable for ICC/IF.
  • ターゲット情報

    • 関連性CNAP1/hCAP-D2 is a regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condensed chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. It may target the condensin complex to DNA via its C-terminal domain (referenced from Swissprot).
    • 細胞内局在Nuclear and cytoplasmic.
    • 参照データベース
    • 別名
      • 2810406C15Rik antibody
      • 2810465G24Rik antibody
      • CAP-D2 antibody
      • CAPD2 antibody
      • CEP2 antibody
      • CEP250 antibody
      • Chromosome condensation-related SMC-associated protein 1 antibody
      • Chromosome-associated protein D2 antibody
      • CNAP1 antibody
      • CND1 antibody
      • Condensin antibody
      • Condensin complex subunit 1 antibody
      • eg7 antibody
      • hCAP-D2 antibody
      • hCAPD2 antibody
      • KIAA0159 antibody
      • mKIAA0159 antibody
      • NCAPD2 antibody
      • non-SMC condensin I complex, subunit D2 antibody
      • XCAP D2 homolog antibody
      see all

    Anti-CNAP1 antibody 画像

    • Lane 1 : Marker
      Lanes 2 - 4 : Anti-CNAP1 antibody (ab34338) at 1 µg/ml

      Lane 1 : As above
      Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
      Lane 3 : Jurkat whole cell lysate (ab7899) at 20 µg
      Lane 4 : A431 whole cell lysate (ab7909) at 20 µg

      Secondary
      Lanes 2 - 4 : IR Dye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution


      Performed under reducing conditions.

      Predicted band size : 157 kDa
      Observed band size : 150 kDa (why is the actual band size different from the predicted?)
    • Image courtesy of Human Protein Atlas ab34338 staining CNAP1 in human testis, showing a predominantly nuclear staining pattern in cells of the seminiferous tubule. Paraffin embedded human testis tissue was incubated with ab34338 (1/100 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

      ab34338 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues. Further results for this antibody can be found at www.proteinatlas.org

    Anti-CNAP1 antibody (ab34338) 使用論文

    ab34338 has not yet been referenced specifically in any publications.

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    Thank you for contacting us. Your credit note ID is XXXXXX for ab34338 and XXXXXX for ab4448.  I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The cre...

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    Thank you for confirming the details. The antibodies should be working fine with the protocol details you have provided. I am not sure why these antibodies fail. I am happy in sending replacement vials of both of these antibodies. Could you let me ...

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    Thank you for your enquiry regarding ab4448 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody. Ab4448 is a very high characterized antibody which has b...

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    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Fixative Paraformaldehyde
    Username

    Dr. Kirk McManus

    Verified customer

    投稿 Mar 05 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"