製品の概要

  • 製品名Anti-CMT antibody
    CMT 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to CMT
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Vector coding for a partial recombinant fusion protein, corresponding to C terminal amino acids 185-284 of Human CMT. Target sequence used to make antibody:FNHVVQNEKS DTHTLVTSGV YAWFRHPSYV GWFYWSIGTQ VMLCNPICGV SYALTVWRFF RDRTEEEEIS LIHFFGEEYL EYKKRVPTGL PFIKGVKVDL.

  • 特記事項


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 50% Glycerol, Whole serum
  • 精製度Whole antiserum
  • 一次抗体 備考This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab52738 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 32 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

ターゲット情報

  • 機能Catalyzes the post-translational methylation of isoprenylated C-terminal cysteine residues.
  • 組織特異性Ubiquitously expressed. Expressed at higher levels in the cerebellum and putamen than in other brain regions. Abundant expression seen in the Purkinje cells and pontine neurons.
  • 配列類似性Belongs to the isoprenylcysteine O-methyltransferase family.
  • 細胞内局在Endoplasmic reticulum membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 1700008E11Rik antibody
    • C80758 antibody
    • fcmt antibody
    • Gm13095 antibody
    • HSTE14 antibody
    • Icmt antibody
    • ICMT_HUMAN antibody
    • Isoprenylcysteine carboxylmethyltransferase antibody
    • MGC118506 antibody
    • MGC39955 antibody
    • MGC95322 antibody
    • MST098 antibody
    • MSTP098 antibody
    • OTTMUSG00000010406 antibody
    • pcCMT antibody
    • PCMT antibody
    • PPMT antibody
    • Prenylated protein carboxyl methyltransferase antibody
    • Prenylcysteine carboxyl methyltransferase antibody
    • Protein-S-isoprenylcysteine O-methyltransferase antibody
    • RP23-421E12.4 antibody
    • STE14 antibody
    see all

Anti-CMT antibody 画像

  • All lanes : Anti-CMT antibody (ab52738) at 1/1000 dilution

    Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a tagged fusion protein of an irrelevant antigen
    Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the antigen (tagged fusion protein)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lane 1 : Rabbit anti-mouse IgG + IgM, horseradish peroxidase conjugated, at 1/5000 dilution
    Lane 2 : Rabbit anti-mouse IgG + IgM, horseradish peroxidase conjugated at 1/5000 dilution


    Predicted band size : 32 kDa

Anti-CMT antibody (ab52738) 使用論文

ab52738 has not yet been referenced specifically in any publications.

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