製品の概要

  • 製品名Anti-Clusterin antibody [CLI-9]
    Clusterin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [CLI-9] to Clusterin
  • アプリケーション適用あり: Flow Cyt, IHC-P, ICC, IHC-Fr, IP, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length native protein (purified) corresponding to Human Clusterin .

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab16077 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB 1/2000. Predicted molecular weight: 54 kDa.

ターゲット情報

  • 機能Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation.
  • 組織特異性Detected in blood plasma, cerebrospinal fluid, milk, seminal plasma and colon mucosa. Detected in the germinal center of colon lymphoid nodules and in colon parasympathetic ganglia of the Auerbach plexus (at protein level). Ubiquitous. Detected in brain, testis, ovary, liver and pancreas, and at lower levels in kidney, heart, spleen and lung.
  • 配列類似性Belongs to the clusterin family.
  • 翻訳後修飾Isoform 1 is proteolytically cleaved on its way through the secretory system, probably within the Golgi lumen.
    Polyubiquitinated, leading to proteasomal degradation.
    Heavily N-glycosylated. About 30% of the protein mass is comprised of complex N-linked carbohydrate.
  • 細胞内局在Secreted. Can retrotranslocate from the secretory compartments to the cytosol upon cellular stress and Nucleus. Cytoplasm. Mitochondrion membrane. Cytoplasm, cytosol. Microsome. Endoplasmic reticulum. Cytoplasmic vesicle, secretory vesicle, chromaffin granule. Isoforms lacking the N-terminal signal sequence have been shown to be cytoplasmic and/or nuclear. Secreted isoforms can retrotranslocate from the secretory compartments to the cytosol upon cellular stress. Detected in perinuclear foci that may be aggresomes containing misfolded, ubiquitinated proteins. Detected at the mitochondrion membrane upon induction of apoptosis.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 40 antibody
    • AAG 4 antibody
    • AAG4 antibody
    • Aging associated protein 4 antibody
    • Aging-associated gene 4 protein antibody
    • AI893575 antibody
    • APO J antibody
    • Apo-J antibody
    • APOJ antibody
    • ApoJalpha antibody
    • ApoJbeta antibody
    • Apolipoprotein J antibody
    • ApolipoproteinJ antibody
    • CLI antibody
    • CLU antibody
    • CLU1 antibody
    • CLU2 antibody
    • CLUS_HUMAN antibody
    • Clusterin alpha chain antibody
    • Clusterin antibody
    • Clusterin beta chain antibody
    • Complement associated protein SP 40 40 antibody
    • Complement associated protein SP 40 antibody
    • Complement associated protein SP40 antibody
    • Complement cytolysis inhibitor a chain antibody
    • Complement cytolysis inhibitor antibody
    • Complement cytolysis inhibitor b chain antibody
    • Complement lysis inhibitor antibody
    • Complement-associated protein SP-40 antibody
    • D14Ucla3 antibody
    • Dimeric acid glycoprotein antibody
    • Glycoprotein 80 antibody
    • Glycoprotein III antibody
    • GP80 antibody
    • Ku70-binding protein 1 antibody
    • KUB 1 antibody
    • KUB1 antibody
    • MGC24903 antibody
    • NA1/NA2 antibody
    • RATTRPM2B antibody
    • SGP 2 antibody
    • SGP2 antibody
    • SP 40 antibody
    • SP40 antibody
    • Sugp-2 antibody
    • Sulfated glycoprotein 2 antibody
    • Testosterone repressed prostate message 2 antibody
    • Testosterone-repressed prostate message 2 antibody
    • TRPM 2 antibody
    • TRPM-2 antibody
    • TRPM2 antibody
    • TRPM2B antibody
    • Trpmb antibody
    see all

Anti-Clusterin antibody [CLI-9] 画像

  • Overlay histogram showing HeLa cells stained with ab16077 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16077, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-Clusterin antibody [CLI-9] (ab16077) 使用論文

This product has been referenced in:
  • Peng L  et al. Serum proteomics analysis and comparisons using iTRAQ in the progression of hepatitis B. Exp Ther Med 6:1169-1176 (2013). WB ; Human . Read more (PubMed: 24223640) »
  • Sinha R  et al. Selenium-Responsive Proteins in the Sera of Selenium-Enriched Yeast-Supplemented Healthy African American and Caucasian Men. Cancer Epidemiol Biomarkers Prev : (2010). WB ; Human . Read more (PubMed: 20643827) »

See all 5 Publications for this product

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I am glad to hear that another product has worked for you. However, it does mean that the ab16077 that you purchased earlier may be faulty. In which case, I am more than happy to offer you a free primary...

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You are correct that "reducing condition" means adding the BME or DTT so that the S-S bonds are cleaved. However, "denaturing condition" actually means boiling, where the protei...

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Thank you for your enquiry.



Firstly, regarding the expected molecular weight (54kDa) stated on the datasheet, the information was taken from SwissProt database (http://www.uniprot.org/uniprot/P10909). As you may already know, the p...

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That is super news. I am really pleased the alternative product worked well. As you have the images available I wonder whether you would like to submit an Abreview for ab69644? We currently have no Abreviews so will be eligible for extra points. Ab...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Human Brain, parafin embedded)
Specification Human Brain, parafin embedded
Fixative Paraformaldehyde
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
Username

Dr. Ruma Raha-Chowdhury

Verified customer

投稿 May 19 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - other (Human Plasma and Follicular Fluid)
Loading amount 18 µg
Specification Human Plasma and Follicular Fluid
Gel Running Conditions Reduced Denaturing (12% gel, Biorad II xi, 16 x 16 cm gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 May 21 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - other (Human plasma 1 in 4 dilution)
Loading amount 20 µg
Specification Human plasma 1 in 4 dilution
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5 %
Username

Abcam user community

Verified customer

投稿 Feb 21 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"