製品の概要

  • 製品名Anti-Clusterin antibody
    Clusterin 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to Clusterin
  • 特異性Typically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA. This antibody reacts with human apoLipoprotein J and has negligible cross-reactivity with Type A-I, A-II, B, C-I, C-II, C-III and E apoLipoproteins.
  • アプリケーション適用あり: Dot blot, ELISA, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length native protein (purified) corresponding to Clusterin .

  • 特記事項This antibody has been used to determine that atherosclerotic lesions in the human aorta contain considerable amounts of lipoproteins. These lipoproteins were observed to be complexed with components of the extracellular matrix (especially LDL and proteoglycans). The role of these matrix-lipoprotein complexes is not entirely clear, however, animal models of atherosclerosis have shown that increased cellular proliferation and increased production of extracellular matrix components occur following injury to the intimal layer of the aorta.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab7621 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Dot blot 1/5000 - 1/10000.
ELISA 1/4000 - 1/8000.
WB Use at an assay dependent dilution.

ターゲット情報

  • 機能Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation.
  • 組織特異性Detected in blood plasma, cerebrospinal fluid, milk, seminal plasma and colon mucosa. Detected in the germinal center of colon lymphoid nodules and in colon parasympathetic ganglia of the Auerbach plexus (at protein level). Ubiquitous. Detected in brain, testis, ovary, liver and pancreas, and at lower levels in kidney, heart, spleen and lung.
  • 配列類似性Belongs to the clusterin family.
  • 翻訳後修飾Isoform 1 is proteolytically cleaved on its way through the secretory system, probably within the Golgi lumen.
    Polyubiquitinated, leading to proteasomal degradation.
    Heavily N-glycosylated. About 30% of the protein mass is comprised of complex N-linked carbohydrate.
  • 細胞内局在Secreted. Can retrotranslocate from the secretory compartments to the cytosol upon cellular stress and Nucleus. Cytoplasm. Mitochondrion membrane. Cytoplasm, cytosol. Microsome. Endoplasmic reticulum. Cytoplasmic vesicle, secretory vesicle, chromaffin granule. Isoforms lacking the N-terminal signal sequence have been shown to be cytoplasmic and/or nuclear. Secreted isoforms can retrotranslocate from the secretory compartments to the cytosol upon cellular stress. Detected in perinuclear foci that may be aggresomes containing misfolded, ubiquitinated proteins. Detected at the mitochondrion membrane upon induction of apoptosis.
  • Information by UniProt
  • 参照データベース
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Anti-Clusterin antibody (ab7621) 使用論文

This product has been referenced in:
  • Christoffersen C  et al. Isolation and characterization of human apolipoprotein M-containing lipoproteins. J Lipid Res 47:1833-43 (2006). WB . Read more (PubMed: 16682745) »

See 1 Publication for this product

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Unfortunately we have not determined the binding affinity of this antibody. I am sorry we are unable to provide any data.

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With regards to volume of antibody, this can be worked out using laboratory math from the information provided on the datasheet:

The concentration of the antib...

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Thank you for your enquiry. I have been in touch with the source of this antibody and unfortunately ab7621 Apolipoprotein J antibody has NOT been tested by IHC. I have removed these details from our datasheet. I am sorry for the confusion caused. U...

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Thank you for your enquiry. Further to your telephone conversation I have contacted the source of this antibody. I have determined that this product is tested by an outside source and therefore unfortunately I do not have details of the testing con...

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Thank you for your enquiry, I'm really sorry about the delay in my reply, I have had to check with the originator if the antibody was tested in ELISA. I can now tell you that it has not yet been tested so I cannot guarantee that it will work in ELISA. ...

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The cross reactivity of this antibody with mouse apo J has not yet been determined.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"