Anti-Clusterin 抗体 (ab39991)
Key features and details
- Goat polyclonal to Clusterin
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Clusterin antibody
Clusterin 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to Clusterin -
由来種
Goat -
特異性
This antibody is expected to recognise both reported isoforms. -
アプリケーション
適用あり: IHC-P, WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human Clusterin (C terminal).
Sequence:(C)EKALQEYRKKHREE
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ポジティブ・コントロール
- WB: Human Colon lysate; ICC: HeLa cells; IHC-P: Human tonsil tissue section.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.05% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab39991の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use a concentration of 2.5 µg/ml.
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WB |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 60 kDa).
1 hour primary incubation is recommended for this product. |
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ICC/IF |
Use a concentration of 10 µg/ml.
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特記事項 |
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IHC-P
Use a concentration of 2.5 µg/ml. |
WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 60 kDa). 1 hour primary incubation is recommended for this product. |
ICC/IF
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation. -
組織特異性
Detected in blood plasma, cerebrospinal fluid, milk, seminal plasma and colon mucosa. Detected in the germinal center of colon lymphoid nodules and in colon parasympathetic ganglia of the Auerbach plexus (at protein level). Ubiquitous. Detected in brain, testis, ovary, liver and pancreas, and at lower levels in kidney, heart, spleen and lung. -
配列類似性
Belongs to the clusterin family. -
翻訳後修飾
Isoform 1 is proteolytically cleaved on its way through the secretory system, probably within the Golgi lumen.
Polyubiquitinated, leading to proteasomal degradation.
Heavily N-glycosylated. About 30% of the protein mass is comprised of complex N-linked carbohydrate. -
細胞内局在
Secreted. Can retrotranslocate from the secretory compartments to the cytosol upon cellular stress and Nucleus. Cytoplasm. Mitochondrion membrane. Cytoplasm, cytosol. Microsome. Endoplasmic reticulum. Cytoplasmic vesicle, secretory vesicle, chromaffin granule. Isoforms lacking the N-terminal signal sequence have been shown to be cytoplasmic and/or nuclear. Secreted isoforms can retrotranslocate from the secretory compartments to the cytosol upon cellular stress. Detected in perinuclear foci that may be aggresomes containing misfolded, ubiquitinated proteins. Detected at the mitochondrion membrane upon induction of apoptosis. - Information by UniProt
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参照データベース
- Entrez Gene: 1191 Human
- Omim: 185430 Human
- SwissProt: P10909 Human
- Unigene: 436657 Human
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別名
- 40 antibody
- AAG 4 antibody
- AAG4 antibody
see all
画像
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Immunofluorescent analysis of paraformaldehyde fixed HeLa cells, staining Clusterin with ab39991 at 10µg/ml. Cells were permeablized with 0.15% Triton. DAPI was used as a nuclear counterstain.
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Anti-Clusterin antibody (ab39991) at 0.3 µg/ml + Human Colon lysate, 35 µg protein in RIPA buffer. Primary incubation was 1 hour. Detected by chemiluminescence.
Predicted band size: 60 kDa
データシートおよび資料
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Datasheet download
参考文献 (4)
ab39991 は 4 報の論文で使用されています。
- Islam K et al. Development of a Label-Free Immunosensor for Clusterin Detection as an Alzheimer's Biomarker. Sensors (Basel) 18:N/A (2018). PubMed: 29361679
- Shafie IN et al. The chaperone protein clusterin may serve as a cerebrospinal fluid biomarker for chronic spinal cord disorders in the dog. Cell Stress Chaperones 19:311-20 (2014). PubMed: 23990410
- Chenau J et al. The cell line secretome, a suitable tool for investigating proteins released in vivo by tumors: application to the study of p53-modulated proteins secreted in lung cancer cells. J Proteome Res 8:4579-91 (2009). WB ; Human . PubMed: 19639960
- Choe L et al. 8-plex quantitation of changes in cerebrospinal fluid protein expression in subjects undergoing intravenous immunoglobulin treatment for Alzheimer's disease. Proteomics 7:3651-60 (2007). PubMed: 17880003