製品の概要

  • 製品名Anti-Clusterin antibody
    Clusterin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Clusterin
  • 特異性Recognizes Clusterin.
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length protein corresponding to Human Clusterin .

  • ポジティブ・コントロール
    • Human brain tissue lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab37172 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
IHC-P Use a concentration of 10 µg/ml.

ターゲット情報

  • 機能Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation.
  • 組織特異性Detected in blood plasma, cerebrospinal fluid, milk, seminal plasma and colon mucosa. Detected in the germinal center of colon lymphoid nodules and in colon parasympathetic ganglia of the Auerbach plexus (at protein level). Ubiquitous. Detected in brain, testis, ovary, liver and pancreas, and at lower levels in kidney, heart, spleen and lung.
  • 配列類似性Belongs to the clusterin family.
  • 翻訳後修飾Isoform 1 is proteolytically cleaved on its way through the secretory system, probably within the Golgi lumen.
    Polyubiquitinated, leading to proteasomal degradation.
    Heavily N-glycosylated. About 30% of the protein mass is comprised of complex N-linked carbohydrate.
  • 細胞内局在Secreted. Can retrotranslocate from the secretory compartments to the cytosol upon cellular stress and Nucleus. Cytoplasm. Mitochondrion membrane. Cytoplasm, cytosol. Microsome. Endoplasmic reticulum. Cytoplasmic vesicle, secretory vesicle, chromaffin granule. Isoforms lacking the N-terminal signal sequence have been shown to be cytoplasmic and/or nuclear. Secreted isoforms can retrotranslocate from the secretory compartments to the cytosol upon cellular stress. Detected in perinuclear foci that may be aggresomes containing misfolded, ubiquitinated proteins. Detected at the mitochondrion membrane upon induction of apoptosis.
  • Information by UniProt
  • 参照データベース
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Anti-Clusterin antibody 画像

  • Immunohistochemistry of Clusterin in human brain tissue with ab37172 at 5 µg/mL.

  • Lane 1 : Anti-Clusterin antibody (ab37172) at 0.5 µg/ml
    Lane 2 : Anti-Clusterin antibody (ab37172) at 1 µg/ml

    Lane 1 : Human brain tissue lysate
    Lane 2 : Human brain tissue lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    anti-rabbit IgG HRP at 1/10000 dilution

    Predicted band size : 60 kDa
    Observed band size : 60 kDa
  • ab37172 at 10 µg/ml staining Apolipoprotein J in human brain tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).

Anti-Clusterin antibody (ab37172) 使用論文

ab37172 has not yet been referenced specifically in any publications.

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