Anti-Clostridium botulinum Toxin B 抗体 [GR-3G7] (ab59715)

製品の概要

  • 製品名
    Anti-Clostridium botulinum Toxin B antibody [GR-3G7]
  • 製品の詳細
    Mouse monoclonal [GR-3G7] to Clostridium botulinum Toxin B
  • 特異性
    This antibody reacts with Clostridium botulinum Toxin B.
  • アプリケーション
    適用あり: Flow Cyt, Competitive ELISAmore details
  • 種交差性
    Reacts with Clostridium botulinum.
  • 免疫原

    Genetic immunisation with cDNA encoding Clostridium botulinum Toxin B.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 精製度
    Protein G purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    GR-3G7
  • アイソタイプ
    IgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab59715 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 1.2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Competitive ELISA 1/200 - 1/400.

ターゲット情報

  • 関連性
    Botulinum toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that cleaves the 76 Gln | Phe 77 bond of synaptobrevin 2.
  • 細胞内局在
    Secreted
  • 別名
    • BoNT/B antibody
    • Bontoxilysin B antibody
    • BontoxilysinB antibody
    • bot B antibody
    • botB antibody
    • Botulinum neurotoxin type B antibody
    see all

画像

  • BOSC23 cells were transiently trans-fected with an expression vector encoding either Clostridium botulinum Toxin B (red curve) or an irrelevant protein (control transfectant). Binding of ab59715 was detected with a PE conjugated secondary antibody. A positive signal was obtained only with Clostridium botulinum Toxin B transfected cells.

プロトコール

参考文献

ab59715 has not yet been referenced specifically in any publications.

レビューと Q&A

Thank you for contacting us with your question and for your patience while I have been in touch with the lab about ab59715. This antibody was raised against the light chain and has only been tested for reactivity with the light chain, so it most...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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