Anti-Clostridium botulinum E Toxoid 抗体 [209F2] (ab40787)

製品の概要

  • 製品名
    Anti-Clostridium botulinum E Toxoid antibody [209F2]
  • 製品の詳細
    Mouse monoclonal [209F2] to Clostridium botulinum E Toxoid
  • 特異性
    BoTox-E (2-17) 100%; BoTox-E 100% Does not cross react with BoTox-A, BoTox-B, BoTox-C or BoTox-F (1-16)
  • アプリケーション
    適用あり: ICC/IF, Inhibition Assaymore details
  • 免疫原

    Synthetic peptide analogue of the amino terminal region of BoTox-E.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: Tissue culture supernatant
  • 精製度
    Tissue culture supernatant
  • 特記事項(精製)
    Sterile filtered culture supernatant.
  • ポリ/モノ
    モノクローナル
  • クローン名
    209F2
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab40787 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF
Inhibition Assay
  • 追加情報
    IF: 1/50.
    This product has been found to stain Neuro2A cells treated with GT1b ganglioside prior to the addition of BoTox-E at a dilution of 1:50 using an indirect immunocytochemical staining procedure.

    Inhib: Use at an assay dependent dilution.
    This product has been found to inhibit the entry of BoTox-E to Neuro2A cells treated with GT1b ganglioside prior to the addition of the toxin.

    With this antibody, we have found that blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性
      Clostridium botulinum E Toxoid acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase. It has a non-toxic component which is necessary to maintain toxicity.
    • 細胞内局在
      Secreted
    • 別名
      • BoNT/E antibody
      • Bontoxilysin e antibody
      • Botulinum neurotoxin type e non toxic component antibody
      • Botulinum neurotoxin type e precursor antibody
      • Botulism E toxoid antibody
      • Ent120 antibody
      see all

    Anti-Clostridium botulinum E Toxoid antibody [209F2] (ab40787) 使用論文

    This product has been referenced in:
    • Shu Y  et al. Phosphorylation of SNAP-25 at Ser187 mediates enhancement of exocytosis by a phorbol ester in INS-1 cells. J Neurosci 28:21-30 (2008). Read more (PubMed: 18171919) »

    See 1 Publication for this product

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    There are currently no Abreviews or Questions for ab40787.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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