Anti-Clostridium botulinum A Toxoid 抗体 (ab20641)

製品の概要

  • 製品名Anti-Clostridium botulinum A Toxoid antibody
    Clostridium botulinum A Toxoid 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Clostridium botulinum A Toxoid
  • 特異性Clostridium botulinum Type A Neurotoxin.
  • アプリケーション適用あり: ELISA, WBmore details
  • 免疫原

    Formaldehyde inactivated Type A Neurotoxin (C. botulinum).

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 20% Glycerol, 0.1M PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab20641 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/10000.
WB Use at an assay dependent dilution. Predicted molecular weight: 149 kDa.

ターゲット情報

  • 関連性Clostridium botulinum neurotoxins are zinc-dependent proteases that block the release of acetylcholine from peripheral cholinergic nerve endings by targeting specific membrane proteins including synaptosomal-associated proteins (SNAP), vesicle-associated membrane proteins (VAMP), and syntaxins. Neurotoxin A is characteristically associated with human botulism and known to target and hydrolyse the 197-Gln-|-Arg-198 bond of SNAP-25.
  • 別名
    • Atx antibody
    • BotA antibody
    • BOTOX antibody
    • Botulinum neurotoxin type A antibody
    • Botulism A toxoid antibody
    see all

Anti-Clostridium botulinum A Toxoid antibody (ab20641) 使用論文

This product has been referenced in:
  • Scotcher MC  et al. Epitope characterization and variable region sequence of f1-40, a high-affinity monoclonal antibody to botulinum neurotoxin type a (Hall strain). PLoS ONE 4:e4924 (2009). WB . Read more (PubMed: 19290051) »
  • Scotcher MC  et al. Characterization of the epitope region of F1-2 and F1-5, two monoclonal antibodies to Botulinum neurotoxin type A. Hybridoma (Larchmt) 28:315-25 (2009). WB . Read more (PubMed: 19857112) »

See all 3 Publications for this product

Product Wall

For catalog # ab20641 and ab20786 a direct ELISA was performed. The limit of detection (LOD) with direct ELISA is greater than 1:10,000 on both products with no amplification using OPD (o-phenylenediamine dihydrochloride) development and HRP conjugated...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Clostridium botulinum Purified protein (cell lysate)
Loading amount 5 µg
Specification cell lysate
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. chung sei kim

Verified customer

投稿 Jun 20 2012

Thank you for your phone call today, and I apologize for any miscommunication with my colleague yesterday. Your credit note ID for ab20641 is: ***. Your credit note ID for ab7202 is: ***. I am sorry that these antibodies did not perform as stated on th...

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Thank you for your enquiry. I can confirm that the immunogen for this antibody (ab20641) has not changed. A toxoid is an attenuated/inactivated toxin. The Clostridium botulinum Type A Neurotoxin is inactivated using formaldehyde before it is us...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"