製品の概要

  • 製品名Anti-CLOCK antibody
    KAT13D / CLOCK 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to KAT13D / CLOCK
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    Recombinant fragment corresponding to Mouse KAT13D/ CLOCK aa 400-499 (internal sequence).
    Sequence:

    ADKSQDSGSDNRINTVSLKEALERFDHSPTPSASSRSSRKSSHTAVSDPS STPTKIPTDTSTPPRQHLPAHEKMTQRRSSFSSQSINSQSVGPSLTQPAM

  • 特記事項


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab43106 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 95 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

ターゲット情報

  • 機能ARNTL/2-CLOCK heterodimers activate E-box element (3'-CACGTG-5') transcription of a number of proteins of the circadian clock. Activates transcription of PER1 and PER2. This transcription is inhibited in a feedback loop by PER and CRY proteins. Has intrinsic histone acetyltransferase activity and this enzymatic function contributes to chromatin-remodeling events implicated in circadian control of gene expression (By similarity). Acetylates primarily histones H3 and H4 (By similarity). Acetylates also a non-histone substrate: ARNTL.
  • 組織特異性Expressed in all tissues examined including spleen, thymus, prostate, testis, ovary, small intestine, colon, leukocytes, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in testis and skeletal muscle. Low levels in thymus, lung and liver. Expressed in all brain regions with highest levels in cerebellum. Highly expressed in the suprachiasmatic nucleus (SCN).
  • 配列類似性Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 PAC (PAS-associated C-terminal) domain.
    Contains 2 PAS (PER-ARNT-SIM) domains.
  • 翻訳後修飾Phosphorylation is dependent on CLOCK-ARNTL heterodimer formation.
  • 細胞内局在Cytoplasm. Nucleus. Shuffling between the cytoplasm and the nucleus is under circadian regulation and is ARNTL-dependent. Phosphorylated form located in the nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • bHLHe8 antibody
    • Circadian locomoter output cycles kaput protein antibody
    • Circadian locomoter output cycles protein kaput antibody
    • Circadian Locomotor Output Cycles Kaput antibody
    • Circadium Locomotor Output Cycles Kaput antibody
    • Class E basic helix-loop-helix protein 8 antibody
    • CLOCK antibody
    • Clock circadian regulator antibody
    • Clock homolog antibody
    • Clock protein antibody
    • CLOCK_HUMAN antibody
    • hCLOCK antibody
    • KIAA0334 antibody
    see all

Anti-CLOCK antibody 画像

  • All lanes : Anti-CLOCK antibody (ab43106) at 1/1000 dilution

    Lane 1 : a total protein extract from E coli with 50ng to 100ng of a tagged fusion protein of an irrelevant antigen
    Lane 2 : a total protein extract from E coli with 50ng to 500ng of the antigen (tagged fusion protein)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Rabbit anti-mouse IgG + IgM (H+L), HRP conjugated, at 1/5000 dilution

    Predicted band size : 95 kDa

Anti-CLOCK antibody (ab43106) 使用論文

This product has been referenced in:
  • Eckle T  et al. Adora2b-elicited Per2 stabilization promotes a HIF-dependent metabolic switch crucial for myocardial adaptation to ischemia. Nat Med 18:774-82 (2012). Mouse . Read more (PubMed: 22504483) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"