製品の概要

  • 製品名Anti-CLK2 antibody
    CLK2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CLK2
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human, Chinese Hamster
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human CLK2.

    (Peptide available as ab99082.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in HeLa whole cell lysate.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab86147 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates serines, threonines and tyrosines.
  • 配列類似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily.
    Contains 1 protein kinase domain.
  • 翻訳後修飾Autophosphorylates on all three types of residues.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CDC like kinase 2 antibody
    • CDC-like kinase 2 antibody
    • CLK 2 antibody
    • CLK kinase antibody
    • CLK2 antibody
    • Clk2 Scamp3 antibody
    • CLK2_HUMAN antibody
    • Dual specificity protein kinase CLK2 antibody
    • hCLK 2 antibody
    • hCLK2 antibody
    • Tu52 antibody
    see all

Anti-CLK2 antibody 画像

  • ab86147 staining CLK2 in human colon carcinoma cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol and blocked with 1% BSA for 30 minutes at 37°C. Samples were incubated with primary antibody (1/30 in PBS + 1% BSA) for 16 hour at 4°C. A FITC-conjugated goat anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.

    See Abreview

  • Anti-CLK2 antibody (ab86147) at 0.5 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 60 kDa
    Observed band size : 60 kDa
    Additional bands at : 36 kDa,64 kDa,94 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes
    Human Dual specificity protein kinase CLK2 contains a number of potential phosphorylation sites (SwissProt) which may explain the banding pattern at a higher molecular weight than predicted (64 kDa).
  • ICC/IF image of ab86147 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab86147 at 5ug overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti- Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in HeLa pfa fixed cell types at 5ug/ml

Anti-CLK2 antibody (ab86147) 使用論文

This product has been referenced in:
  • Petsalaki E & Zachos G Clks 1, 2 and 4 prevent chromatin breakage by regulating the Aurora B-dependent abscission checkpoint. Nat Commun 7:11451 (2016). Read more (PubMed: 27126587) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 50 µg
Gel Running Conditions Non-reduced Denaturing (10)
Sample Human Cell lysate - whole cell (Colon carcinoma cells)
Specification Colon carcinoma cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Eleni Petsalaki

Verified customer

投稿 Apr 18 2014

Application Western blot
Loading amount 50 µg
Gel Running Conditions Non-reduced Denaturing (10)
Sample Hamster Cell lysate - whole cell (Chinese Hamster Ovary cells)
Specification Chinese Hamster Ovary cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5µg/mL · Temperature: 25°C
Username

Dr. Eleni Petsalaki

Verified customer

投稿 Apr 02 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 37°C
Sample Hamster Cell (Chinese Hamster Ovary cells)
Specification Chinese Hamster Ovary cells
Permeabilization Yes - 0.5% Triton
Fixative Methanol
Username

Dr. Eleni Petsalaki

Verified customer

投稿 Apr 01 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 37°C
Sample Human Cell (Colon carcinoma cells)
Specification Colon carcinoma cells
Permeabilization No
Fixative Methanol
Username

Dr. Eleni Petsalaki

Verified customer

投稿 Mar 24 2014

I am very pleased to hear you would like to accept our offer and test ab65082 and/or ab86147 in immunoprecipitation. This code will give you one free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for immun...

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