The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 75 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
機能May insert into membranes and form chloride ion channels. May play a critical role in water-secreting cells, possibly through the regulation of chloride ion transport.
組織特異性Expressed in brain, placenta, pancreas and liver.
配列類似性Belongs to the chloride channel CLIC family. Contains 1 GST C-terminal domain.
ドメインMembers of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion.
細胞内局在Cytoplasm. Cell membrane. Predominantly cytoplasmic. Upon chloride ion efflux from the cell, it is translocated to the plasma membrane.