製品の概要

  • 製品名Anti-CLIC5 antibody
    CLIC5 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CLIC5
  • アプリケーション適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant human CLIC5 protein

  • ポジティブ・コントロール
    • K562 cell line lysate. Human colon carcinoma tissue.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度Ammonium Sulphate Precipitation
  • 特記事項(精製)ab75948 is prepared by Saturated Ammonium Sulfate precipitation followed by dialysis against PBS.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab75948 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/50 - 1/100. Predicted molecular weight: 47 kDa.
ELISA 1/1000.
IHC-P 1/10 - 1/50.

ターゲット情報

  • 機能Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. May play a role in the regulation of transepithelial ion absorption and secretion. Required for normal formation of stereocilia in the inner ear and normal development of the organ of Corti (By similarity). Is required for the development and/or maintenance of the proper glomerular endothelial cell and podocyte architecture.
  • 組織特異性Isoform 1: Expressed in renal glomeruli endothelial cells and podocytes (at protein level).
  • 配列類似性Belongs to the chloride channel CLIC family.
    Contains 1 GST C-terminal domain.
  • ドメインMembers of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion.
  • 細胞内局在Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Membrane. Associates with the cortical actin cytoskeleton. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain and Golgi apparatus. Cytoplasm > cytoskeleton > centrosome. Membrane. Colocalized with AKAP9 at the Golgi apparatus as well as, to a lesser extent, the centrosome. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain (By similarity). Colocalized with podocalyxin in renal glomeruli.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Chloride intracellular channel 5 antibody
    • Chloride intracellular channel 5A antibody
    • Chloride intracellular channel protein 5 antibody
    • CLIC 5 antibody
    • CLIC 5B antibody
    • CLIC5 antibody
    • CLIC5_HUMAN antibody
    • CLIC5B antibody
    • dJ447E21.4 antibody
    • FLJ90663 antibody
    • MST 130 antibody
    • MST130 antibody
    • MSTP 130 antibody
    • MSTP130 antibody
    see all

Anti-CLIC5 antibody 画像

  • Anti-CLIC5 antibody (ab75948) at 1/60 dilution + K562 cell line lysate at 35 µg

    Predicted band size : 47 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.
  • ab75948, at 1/10 dilution, staining CLIC5 in formalin-fixed, paraffin-embedded human colon carcinoma tissue by Immunohistochemistry. ab75948 was peroxidase conjugated to the secondary antibody, followed by DAB staining.

Anti-CLIC5 antibody (ab75948) 使用論文

ab75948 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"