製品の概要

  • 製品名Anti-CLIC4 antibody
    CLIC4 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to CLIC4
  • アプリケーション適用あり: WB, ELISA, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Cow, Dog
  • 免疫原

    Synthetic peptide:

    NGLKEEDKEPLIE-C

    , corresponding to N terminal amino acids 8-20 of Human CLIC4 (NP_039234.1).

  • ポジティブ・コントロール
    • Human kidney and human placenta lysates. Min6 cells transiently expressing mouse CLIC4.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab77626 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
ICC/IF
  • 追加情報Peptide ELISA: Antibody detection limit dilution 1/64000.
    ICC/IF: Use at a concentration of 5 µg/ml with overnight incubation.
    WB: Use at a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Promotes cell-surface expression of HRH3. Has alternate cellular functions like a potential role in angiogenesis or in maintaining apical-basolateral membrane polarity during mitosis and cytokinesis. Could also promote endothelial cell proliferation and regulate endothelial morphogenesis (tubulogenesis).
    • 組織特異性Detected in epithelial cells from colon, esophagus and kidney (at protein level). Expression is prominent in heart, kidney, placenta and skeletal muscle.
    • 配列類似性Belongs to the chloride channel CLIC family.
      Contains 1 GST C-terminal domain.
    • ドメインMembers of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion.
    • 細胞内局在Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasmic vesicle membrane. Nucleus matrix. Cell membrane. Mitochondrion. Cell junction. Colocalized with AKAP9 at the centrosome and midbody. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain. Present in an intracellular vesicular compartment that likely represent trans-Golgi network vesicles.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Chloride intracellular channel 4 antibody
      • Chloride intracellular channel 4 (mitochondrial) antibody
      • Chloride intracellular channel 4 like antibody
      • Chloride intracellular channel protein 4 antibody
      • Clic4 antibody
      • CLIC4_HUMAN antibody
      • CLIC4L antibody
      • DKFZP566G223 antibody
      • FLJ38640 antibody
      • H1 antibody
      • HUH1 antibody
      • Intracellular chloride ion channel protein p64H1 antibody
      • MC3S5 antibody
      • mtCLIC antibody
      • p64H1 antibody
      see all

    Anti-CLIC4 antibody 画像

    • Anti-CLIC4 antibody (ab77626) at 0.1 µg/ml + human kidney lysate in RIPA buffer at 35 µg

      Predicted band size : 29 kDa
      Observed band size : 29 kDa
    • Immunofluorescent staining of min6 cells transiently expressing mouse CLIC4 using ab77626 at 5µg/ml with overnight incubation. Nuclear counter staining by Hoechst.

    Anti-CLIC4 antibody (ab77626) 使用論文

    ab77626 has not yet been referenced specifically in any publications.

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    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab76593.

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