製品の概要

  • 製品名Anti-CLIC3 antibody
    CLIC3 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to CLIC3
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length human CLIC3 protein (NP_004660.2).

  • ポジティブ・コントロール
    • WB: Human pancreas tissue lysate, A-431 cell lysate, CLIC3 transfected 293T cell lysate ICC/IF: HeLa cell

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab89199 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 27 kDa.
ICC/IF Use a concentration of 10 µg/ml.

ターゲット情報

  • 関連性Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 3 is a member of the p64 family and is predominantly localized in the nucleus and stimulates chloride ion channel activity. In addition, this protein may participate in cellular growth control, based on its association with ERK7, a member of the MAP kinase family.
  • 細胞内局在Nuclear, cytoplasmic and Single pass membrane. Predominantly nuclear but also found in the cytoplasm. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain
  • 参照データベース
  • 別名
    • 2300003G24Rik antibody
    • Chloride intracellular channel 3 antibody
    • Chloride intracellular channel protein 3 antibody
    • MGC109225 antibody
    • OTTHUMP00000022630 antibody
    see all

Anti-CLIC3 antibody 画像

  • Anti-CLIC3 antibody (ab89199) at 1 µg/ml + human pancreas tissue lysate at 50 µg
    Developed using the ECL technique

    Predicted band size : 27 kDa
    Observed band size : 24 kDa (why is the actual band size different from the predicted?)
  • Anti-CLIC3 antibody (ab89199) at 1 µg/ml + A-431 cell lysate at 50 µg
    Developed using the ECL technique

    Predicted band size : 27 kDa
    Observed band size : 33 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-CLIC3 antibody (ab89199) at 1 µg/ml

    Lane 1 : CLIC3 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Developed using the ECL technique

    Predicted band size : 27 kDa
    Observed band size : 26 kDa (why is the actual band size different from the predicted?)
  • ab89199, at 10 µg/ml, staining CLIC3 in HeLa cell by Immunofluorescence.

Anti-CLIC3 antibody (ab89199) 使用論文

ab89199 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"