製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to CLIC1
  • 特異性
    ab28722 recognises Chloride intracellular channel protein 1(CLIC1)
  • アプリケーション
    適用あり: ELISA, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Dog, Pig, Zebrafish
  • 免疫原

    Synthetic peptide derived from a region between amino acids 62-111 of human CLIC1. (Note: The exact immunogen sequence is proprietary).

  • ポジティブ・コントロール
    • Jurkat cell lysate, Human kidney tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab28722 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/312500.
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 27 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Can insert into membranes and form chloride ion channels. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Involved in regulation of the cell cycle.
  • 組織特異性
    Expression is prominent in heart, placenta, liver, kidney and pancreas.
  • 配列類似性
    Belongs to the chloride channel CLIC family.
    Contains 1 GST C-terminal domain.
  • ドメイン
    Members of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion.
  • 翻訳後修飾
    Hydrogen peroxide treatment causes a conformation change, leading to dimerization and formation of an intramolecular disulfide bond between Cys-24 and Cys-59.
  • 細胞内局在
    Nucleus. Nucleus membrane. Cytoplasm. Cell membrane. Mostly in the nucleus including in the nuclear membrane. Small amount in the cytoplasm and the plasma membrane. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Chloride channel ABP antibody
    • Chloride intracellular channel 1 antibody
    • Chloride intracellular channel protein 1 antibody
    • CLIC 1 antibody
    • CLIC1 antibody
    • CLIC1_HUMAN antibody
    • G6 antibody
    • hRNCC antibody
    • NCC 27 antibody
    • NCC27 antibody
    • Nuclear chloride ion channel 27 antibody
    • Nuclear chloride ion channel protein antibody
    • p64CLCP antibody
    • Regulatory nuclear chloride ion channel protein antibody
    • RNCC protein antibody
    see all

画像

  • Anti-CLIC1 antibody (ab28722) at 1.25 µg/ml + Jurkat cell lysate

    Secondary
    HRP conjugated anti-Rabbit IgG 1: 50,000 - 100,000

    Predicted band size : 27 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry analysis of human kidney tissue labelling CLIC1 with ab28722.

参考文献

This product has been referenced in:
  • Zhou J  et al. Proteomics-Based Identification and Analysis of Proteins Associated with Helicobacter pylori in Gastric Cancer. PLoS One 11:e0146521 (2016). WB . Read more (PubMed: 26745502) »
  • Chen WQ  et al. Variations of protein levels in human amniotic fluid stem cells CD117/2 over passages 5-25. J Proteome Res 8:5285-95 (2009). Read more (PubMed: 19791749) »

See all 2 Publications for this product

レビューと Q&A

Application
Western blot
Sample
Human Purified protein (Human CLIC1 and CLIC4 full length protein)
Gel Running Conditions
Reduced Denaturing (12% SDS gel)
Loading amount
3 µg
Specification
Human CLIC1 and CLIC4 full length protein
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Jul 18 2015

Application
Immunoprecipitation
Immuno-precipitation step
Other - Pierce Protein A/G Magnetic Beads
Sample
Human Cell lysate - whole cell (MCF7)
Specification
MCF7
Total protein in input
250 µg
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投稿 May 28 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (astrocytoma)
Loading amount
10 µg
Specification
astrocytoma
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
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投稿 Apr 09 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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