製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to CLEC9A
  • アプリケーション
    適用あり: WB, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human CLEC9A.

    (Peptide available as ab92420.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in human spleen and kidney tissue lysates.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab79661 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
Flow Cyt
  • 追加情報
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 27 kDa).
    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Functions as an endocytic receptor on a small subset of myeloid cells specialized for the uptake and processing of material from dead cells. Recognizes filamentous form of actin in association with particular actin-binding domains of cytoskeletal proteins, including spectrin, exposed when cell membranes are damaged, and mediate the cross-presentation of dead-cell associated antigens in a Syk-dependent manner.
    • 組織特異性
      In peripheral blood highly restricted on the surface of BDCA31(+) dendritic cells and on a small subset of CD14(+) and CD16(-) monocytes.
    • 配列類似性
      Contains 1 C-type lectin domain.
    • 翻訳後修飾
      N-glycosylated.
    • 細胞内局在
      Membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • C type lectin domain family 9, member A antibody
      • C-type lectin domain family 9 member A antibody
      • CLC9A_HUMAN antibody
      • CLEC9A antibody
      • DNGR1 antibody
      • HEEE9341 antibody
      • PRO34046 antibody
      • UNQ9341 antibody
      see all

    画像

    • All lanes : Anti-CLEC9A antibody (ab79661) at 1 µg/ml

      Lane 1 : Human spleen tissue lysate - total protein (ab29699)
      Lane 2 : Human kidney tissue lysate - total protein (ab30203)

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 27 kDa
      Observed band size : 33 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 17 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 3 minutes

    参考文献

    This product has been referenced in:
    • Schreibelt G  et al. The C-type lectin receptor CLEC9A mediates antigen uptake and (cross-)presentation by human blood BDCA3+ myeloid dendritic cells. Blood 119:2284-92 (2012). Flow Cyt ; Human . Read more (PubMed: 22234694) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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