製品の概要

  • 製品名Anti-CLEC2D antibody
    CLEC2D 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to CLEC2D
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    Synthetic peptide:

    CVTKASLPMLSPTGSPQ

    , corresponding to amino acids 2-18 of CLEC2D (OCIL).

  • ポジティブ・コントロール
    • Mouse spleen cell lysates.
  • 特記事項


    Recently, Zhou et al have identified a protein that can inhibit multinucleate Osteoclast formation in murine osteoblast and spleen cell co-cultures. This protein called, Osteoclast Inhibitory Lectin (OCIL) can also inhibit osteoclast formation in spleen cell cultures treated with RANKL and M-CSF. Furthermore, mOCIL acted directly on macrophage/monocyte cells as evidenced by its inhibitory action on adherent spleen cell cultures, which were depleted of stromal and lymphocytic cells. mOCIL completely inhibited osteoclast formation during the proliferative phase of osteoclast formation and resulted in 70% inhibition during the differentiation phase. Osteoblast OCIL mRNA expression was enhanced by parathyroid hormone, calcitriol, interleukin-1 and IL-11, and retinoic acid. OCIL is expressed in osteoblasts and chondrocytes as well as in a variety of extraskeletal tissues. Since OCIL expression was found to be very similar to distribution for RANKL, it has been suggested that RANKl and OCIL may interact in skeleton and in extracellular tissues.

製品の特性

  • 製品の状態Liquid
  • 保存方法Store at +4°C short term (1-2 weeks) (add antibacterial agent). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度IgG fraction
  • 一次抗体 備考Recently, Zhou et al have identified a protein that can inhibit multinucleate Osteoclast formation in murine osteoblast and spleen cell co-cultures. This protein called, Osteoclast Inhibitory Lectin (OCIL) can also inhibit osteoclast formation in spleen cell cultures treated with RANKL and M-CSF. Furthermore, mOCIL acted directly on macrophage/monocyte cells as evidenced by its inhibitory action on adherent spleen cell cultures, which were depleted of stromal and lymphocytic cells. mOCIL completely inhibited osteoclast formation during the proliferative phase of osteoclast formation and resulted in 70% inhibition during the differentiation phase. Osteoblast OCIL mRNA expression was enhanced by parathyroid hormone, calcitriol, interleukin-1 and IL-11, and retinoic acid. OCIL is expressed in osteoblasts and chondrocytes as well as in a variety of extraskeletal tissues. Since OCIL expression was found to be very similar to distribution for RANKL, it has been suggested that RANKl and OCIL may interact in skeleton and in extracellular tissues.
  • ポリ/モノモノクローナル
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab13664 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 25 kDa.

ターゲット情報

  • 機能Receptor for KLRB1 that protects target cells against natural killer cell-mediated lysis. Inhibits osteoclast formation. Inhibits bone resorption. Modulates the release of interferon-gamma. Binds high molecular weight sulfated glycosaminoglycans.
  • 組織特異性Detected in peripheral blood leukocytes, osteoblasts, lymph node, thymus and spleen. Isoform 1, isoform 2 and isoform 4 are expressed in T and B lymphocytes, and at lower levels in NK cells. They are also expressed in B-cell lines and LPS-matured monocyte-derived dendritic cells.
  • 配列類似性Contains 1 C-type lectin domain.
  • 細胞内局在Endoplasmic reticulum and Cell membrane. Cell surface.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C type lectin related f antibody
    • C type lectin superfamily 2, member D antibody
    • C-type lectin domain family 2 member D antibody
    • CLAX antibody
    • CLC2D_HUMAN antibody
    • CLEC2D antibody
    • CLEC2D protein antibody
    • Clec2d5 antibody
    • L LT1 antibody
    • Lectin like NK cell receptor antibody
    • Lectin like transcript 1 antibody
    • Lectin-like NK cell receptor antibody
    • Lectin-like transcript 1 antibody
    • LLT-1 antibody
    • LLT1 antibody
    • OCIL antibody
    • Osteoclast inhibitory lectin antibody
    see all

Anti-CLEC2D antibody 画像

  • Western blot analysis of CLEC2D (OCIL) in mouse spleen cell lysates. Thirty microgram of cell lysates per lane of mini gel was analyzed in Western blotting using ab1664.

Anti-CLEC2D antibody (ab13664) 使用論文

This product has been referenced in:
  • Maruyama Y  et al. The effect of podoplanin inhibition on lymphangiogenesis under pathological conditions. Invest Ophthalmol Vis Sci 55:4813-22 (2014). Read more (PubMed: 24985477) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"