Anti-KACL 抗体 (ab76711)
Key features and details
- Rabbit polyclonal to KACL
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-KACL antibody
KACL 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to KACL -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human KACL aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab103565) -
ポジティブ・コントロール
- This antibody gave a positive signal in the following lysates: Human Spleen Tissue; Human Thymus Tissue; Human Small Intestine Tissue; Human Bone Marrow Tissue; Raji Whole Cell; Jurkat Whole Cell; MOLT4 Whole Cell.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab76711の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 20 kDa).
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 20 kDa). |
ターゲット情報
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機能
Plays a role in modulating the extent of T-cell expansion. Enhances the expansion of TCR-stimulated T cells by increasing their survival through enhanced expression of anti-apoptotic proteins. May modulate the capacity of T-cells to home to lymph nodes through SELL. Facilitates dedicated immune recognition of keratinocytes via interaction with its receptor KLRF2 by stimulating natural killer cell mediated cytotoxicity. -
組織特異性
Mainly expressed in skin. Also expressed in keratinocytes, spleen, thymus, small intestine, peripheral blood monocytes, bone marrow, ovary, testis and skin. High expression in CD8(+), B lymphocytes and naive CD4(+) T cells. Restricted mostly to proliferating lymphocytes. Not detected in myeloid leukocytes or natural killer (NK) cells. -
配列類似性
Contains 1 C-type lectin domain. -
翻訳後修飾
N-glycosylated. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 387836 Human
- Omim: 612087 Human
- SwissProt: Q6UVW9 Human
- Unigene: 527665 Human
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別名
- C-type lectin domain family 2 member A antibody
- CLC2A_HUMAN antibody
- CLEC2A antibody
see all
画像
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All lanes : Anti-KACL antibody (ab76711) at 1 µg/ml
Lane 1 : Human spleen tissue lysate - total protein (ab29699)
Lane 2 : Human thymus tissue lysate - total protein (ab30146)
Lane 3 : Human small intestine tissue lysate - total protein (ab29276)
Lane 4 : Bone Marrow (Human) Tissue Lysate - adult normal tissue
Lane 5 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 6 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 7 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 20 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
Additional bands at: 300 kDa, 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutesKACL contains a potential glycosylation site (SwissProt), which may explain its migration at a higher molecular weight than predicted. The band at 32-kDa is consistent with what has been reported in the literature (PMID:16174766).
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab76711 は論文での使用が確認できていません。