The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
Retinoblastoma gene product (RB) is a tumor suppressor protein and acts as cell cycle regulator. Recently, RB has been shown to play a role in apoptosis. During apoptosis, RB is subjected to cleavage by caspase 3 like protease. Caspase 3 cleaves RB into 48 and 68kDa fragments. Cleavage of RB would make it functionally inactive, causing release of E2F 1. The E2F 1 then may promote apoptosis.
Western blot - Anti-cleaved RB antibody [ 172C1094] (ab13905)
Predicted band size : 68 kDa
Western blot analysis: HL60 cells were treated for with camptothecin for 24 hours. Harvested cells were lysed and resolved by SDS-PAGE and transferred onto membrane. The membrane was stained with ab13905 and immunoreactivity was detected by chemiluminescense.