The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Predicted molecular weight: 85 kDa.
1/50. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
追加情報Is unsuitable for IHC.
機能Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Required for PARP9 and DTX3L recruitment to DNA damage sites. PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites.
翻訳後修飾Phosphorylated by PRKDC and TXK. Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites. S-nitrosylated, leading to inhibit transcription regulation activity.
細胞内局在Nucleus. Nucleus, nucleolus. Localizes at sites of DNA damage.
Western blot - cleaved PARP antibody [Y34] (ab32561)
All lanes : Anti-Cleaved PARP antibody [Y34] (ab32561) at 1/1000 dilution
Lane 1 : Un-treated Jurkat cell lysate. Lane 2 : Jurkat cell lysate treated with Camptothecin.
Predicted band size : 85 kDa Observed band size : 85 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Cleaved PARP antibody [Y34] (ab32561)This image is courtesy of an anonymous Abreview
ab32561 staining Cleaved PARP in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde and permeabilized with 0.5% Triton X-100 in PBS. Samples were incubated with primary antibody (1/500 in PBS) for 1 hour at 22°C. ab150081, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Counterstained with DAPI.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-cleaved PARP antibody [Y34] (ab32561)Image from Wang L et al., PLoS One. 2011;6(9):e24405. Epub 2011 Sep 9. Fig 6.; doi:10.1371/journal.pone.0024405; September 9, 2011, PLoS ONE 6(9): e24405.
Immunohistochemical analysis of OVCAR-3 tumour xenografts in nude mice, staining cleaved PARP with ab32561.
Antigen retrieval was performed via heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100) overnight at 4°C. A biotinyated anti-rabbit IgG (1/150) was used as the secondary antibody and staining was detected using DAB.
Anti-Cleaved PARP antibody [Y34] (ab32561) 使用論文
This product has been referenced in:
Peh J et al. The Combination of Vemurafenib and Procaspase-3 Activation Is Synergistic in Mutant BRAF Melanomas. Mol Cancer Ther15:1859-69 (2016).
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