製品の概要

  • 製品名Anti-Cleaved PARP antibody [Y34]
    Cleaved PARP 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [Y34] to Cleaved PARP
  • 特異性This antibody is specific for p85 cleaved form of PARP.
  • アプリケーション適用あり: WB, Flow Cyt, IP, ICC/IFmore details
    適用なし: IHC
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to residues following the cleavage of site of Human PARP.

  • ポジティブ・コントロール
    • Jurkat cell lysate.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab32561 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 85 kDa.
Flow Cyt 1/50. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP 1/50.
ICC/IF 1/500.
  • 追加情報Is unsuitable for IHC.
  • ターゲット情報

    • 機能Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Required for PARP9 and DTX3L recruitment to DNA damage sites. PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites.
    • 配列類似性Contains 1 BRCT domain.
      Contains 1 PARP alpha-helical domain.
      Contains 1 PARP catalytic domain.
      Contains 2 PARP-type zinc fingers.
    • 翻訳後修飾Phosphorylated by PRKDC and TXK.
      Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
      S-nitrosylated, leading to inhibit transcription regulation activity.
    • 細胞内局在Nucleus. Nucleus, nucleolus. Localizes at sites of DNA damage.
    • Information by UniProt
    • 参照データベース
    • 別名
      • ADP-ribosyltransferase diphtheria toxin-like 1 antibody
      • ADPRT 1 antibody
      • ADPRT antibody
      • ADPRT1 antibody
      • APOPAIN antibody
      • ARTD1 antibody
      • NAD(+) ADP-ribosyltransferase 1 antibody
      • PARP antibody
      • PARP-1 antibody
      • PARP1 antibody
      • PARP1_HUMAN antibody
      • Poly [ADP-ribose] polymerase 1 antibody
      • Poly ADP ribose polymerase 1 antibody
      • Poly[ADP-ribose] synthase 1 antibody
      • PPOL antibody
      • SCA1 antibody
      see all

    Anti-Cleaved PARP antibody [Y34] 画像

    • All lanes : Anti-Cleaved PARP antibody [Y34] (ab32561) at 1/1000 dilution

      Lane 1 : Un-treated Jurkat cell lysate.
      Lane 2 : Jurkat cell lysate treated with Camptothecin.


      Predicted band size : 85 kDa
      Observed band size : 85 kDa
    • ab32561 staining Cleaved PARP in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde and permeabilized with 0.5% Triton X-100 in PBS. Samples were incubated with primary antibody (1/500 in PBS) for 1 hour at 22°C. ab150081, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Counterstained with DAPI.

      See Abreview

    • Immunohistochemical analysis of OVCAR-3 tumour xenografts in nude mice, staining cleaved PARP with ab32561.

      Antigen retrieval was performed via heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100) overnight at 4°C. A biotinyated anti-rabbit IgG (1/150) was used as the secondary antibody and staining was detected using DAB.

    Anti-Cleaved PARP antibody [Y34] (ab32561) 使用論文

    This product has been referenced in:
    • Peh J  et al. The Combination of Vemurafenib and Procaspase-3 Activation Is Synergistic in Mutant BRAF Melanomas. Mol Cancer Ther 15:1859-69 (2016). Read more (PubMed: 27297867) »
    • Gao L  et al. Glycyrrhizic acid alleviates bleomycin-induced pulmonary fibrosis in rats. Front Pharmacol 6:215 (2015). WB ; Rat . Read more (PubMed: 26483688) »

    See all 13 Publications for this product

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Permeabilization Yes - 0.5% Triton-X100 in PBS
    Fixative Formaldehyde
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    投稿 Nov 05 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"