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RabMAb

Anti-Cleaved alpha-fodrin (Asp1185) 抗体 [EPR3830-17] (ab92373)

製品の概要

  • 製品名
    Anti-Cleaved alpha-fodrin (Asp1185) antibody [EPR3830-17]
  • 製品の詳細
    Rabbit monoclonal [EPR3830-17] to Cleaved alpha-fodrin (Asp1185)
  • 特異性
    Only detects the large fragment of Alpha Fodrin resulting from cleavage at aspartic acid 1185.
  • アプリケーション
    適用あり: WB, IPmore details
    適用なし: Flow Cyt,ICC or IHC-P
  • 種交差性
    交差種: Human
  • 免疫原

    corresponding to Human Cleaved alpha-fodrin (Asp1185) aa 1150-1250.

  • ポジティブ・コントロール
    • HeLa + Camptothecin cell lysate, HeLa cell lysate, Jurkat + Staurosporine cell lysate, Jurkat cell lysate
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab92373 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000 - 1/50000. Predicted molecular weight: 285 kDa.
IP 1/10 - 1/100.
  • 追加情報
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • ターゲット情報

    • 機能
      Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
    • 関連疾患
      Epileptic encephalopathy, early infantile, 5
    • 配列類似性
      Belongs to the spectrin family.
      Contains 3 EF-hand domains.
      Contains 1 SH3 domain.
      Contains 23 spectrin repeats.
    • 翻訳後修飾
      Phosphorylation of Tyr-1176 decreases sensitivity to cleavage by calpain in vitro.
    • 細胞内局在
      Cytoplasm, cytoskeleton. Cytoplasm, cell cortex. Expressed along the cell membrane in podocytes and presumptive tubule cells during glomerulogenesis and is expressed along lateral cell margins in tubule cells.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Alpha-II spectrin antibody
      • Fodrin alpha chain antibody
      • non-erythrocytic 1 antibody
      • non-erythroid alpha subunit antibody
      • Spectrin alpha chain antibody
      • Spectrin antibody
      • SPTAN1 antibody
      • SPTN1_HUMAN antibody
      see all

    Anti-Cleaved alpha-fodrin (Asp1185) antibody [EPR3830-17] 画像

    • All lanes : Anti-Cleaved alpha-fodrin (Asp1185) antibody [EPR3830-17] (ab92373)

      Lane 1 : HeLa + Camptothecin cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Jurkat + Staurosporine cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 285 kDa
      Observed band size : 150 kDa (why is the actual band size different from the predicted?)

    Anti-Cleaved alpha-fodrin (Asp1185) antibody [EPR3830-17] (ab92373) 使用論文

    ab92373 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab92373.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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