製品の概要

  • 製品名Anti-Cleavage stimulation factor 2 antibody
    Cleavage stimulation factor 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Cleavage stimulation factor 2
  • アプリケーション適用あり: IHC-P, WB, IP, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rabbit, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residues 375 and 425 of human Cleavage stimulation factor 2 (NP_001316.1)

  • ポジティブ・コントロール
    • HeLa whole cell lysate This antibody gave a positive result when used in the following formaldehyde/methanol fixed cell lines: MCF-7/HepG2. IHC-P: FFPE human testis tissue sections.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab72297 was affinity purified using an epitope specific to CSTF64 immobilized on solid support.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab72297 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 68 kDa (predicted molecular weight: 61 kDa).
IP Use at 2-5 µg/mg of lysate.
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. This subunit is directly involved in the binding to pre-mRNAs.
  • 配列類似性Contains 1 RRM (RNA recognition motif) domain.
  • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Nucleus. Localized with DDX1 in cleavage bodies.
  • Information by UniProt
  • 参照データベース
  • 別名
    • betaCstF 64 variant 2 antibody
    • CF-1 64 kDa subunit antibody
    • CF1 64 kDa subunit antibody
    • Cleavage stimulation factor 64 kDa subunit antibody
    • Cleavage stimulation factor subunit 2 antibody
    • Cleavage stimulation factor, 3' pre RNA, subunit 2, 64kDa antibody
    • CSTF 2 antibody
    • CstF 64 antibody
    • CSTF 64 kDa subunit antibody
    • CstF-64 antibody
    • CSTF2 antibody
    • CSTF2_HUMAN antibody
    • MGC188397 antibody
    see all

Anti-Cleavage stimulation factor 2 antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling Cleavage stimulation factor 2 with ab72297 at 1/1000 (1µg/ml). Detection: DAB.
  • IHC image of Cleavage stimulation factor 2 staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72297, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ab72297 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab72297 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HepG2 cells at 5ug/ml.

  • All lanes : Anti-Cleavage stimulation factor 2 antibody (ab72297) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 61 kDa
    Observed band size : 68 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 10 seconds
  • Detection of human Cleavage stimulation factor 2 by Immunoprecipitation using whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), and ab72297 at 3 µg/mg lysate. Subsequent WB detection was performed using ab72297 at 0.1 µg/ml. Detection was by chemiluminescence with an exposure time of 3 seconds.

Anti-Cleavage stimulation factor 2 antibody (ab72297) 使用論文

This product has been referenced in:
  • Kim C  et al. Regulation of post-translational protein arginine methylation during HeLa cell cycle. Biochim Biophys Acta 1800:977-85 (2010). WB ; Human . Read more (PubMed: 20541591) »

See 1 Publication for this product

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