Anti-Cleavage stimulation factor 2 抗体 (ab64942)

製品の概要

  • 製品名Anti-Cleavage stimulation factor 2 antibody
    Cleavage stimulation factor 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Cleavage stimulation factor 2
  • 特異性Detects endogenous levels of total Cleavage stimulation factor 2 protein.
  • アプリケーション適用あり: WB, ICC/IF, ELISAmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide (Human) from the N-terminus

  • ポジティブ・コントロール
    • 293, HepG2 and Jurkat cell extract. HeLa cells.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab64942 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
ICC/IF 1/500 - 1/1000.
ELISA 1/10000.

ターゲット情報

  • 機能One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. This subunit is directly involved in the binding to pre-mRNAs.
  • 配列類似性Contains 1 RRM (RNA recognition motif) domain.
  • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Nucleus. Localized with DDX1 in cleavage bodies.
  • Information by UniProt
  • 参照データベース
  • 別名
    • betaCstF 64 variant 2 antibody
    • CF-1 64 kDa subunit antibody
    • CF1 64 kDa subunit antibody
    • Cleavage stimulation factor 64 kDa subunit antibody
    • Cleavage stimulation factor subunit 2 antibody
    • Cleavage stimulation factor, 3' pre RNA, subunit 2, 64kDa antibody
    • CSTF 2 antibody
    • CstF 64 antibody
    • CSTF 64 kDa subunit antibody
    • CstF-64 antibody
    • CSTF2 antibody
    • CSTF2_HUMAN antibody
    • MGC188397 antibody
    see all

Anti-Cleavage stimulation factor 2 antibody 画像

  • All lanes : Anti-Cleavage stimulation factor 2 antibody (ab64942) at 1/500 dilution

    Lane 1 : 293 cell extract
    Lane 2 : HepG2 cell extract
    Lane 3 : Jurkat cell extract
    Lane 4 : Jurkat cell extract with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 61 kDa
    Observed band size : 61 kDa
    Additional bands at : 120 kDa,20 kDa,30 kDa,48 kDa,80 kDa. We are unsure as to the identity of these extra bands.
  • Immunofluorescence analysis of HeLa cells, using ab64942 at 1/500 dilution. Right hand panel was also treated with immunising peptide.

Anti-Cleavage stimulation factor 2 antibody (ab64942) 使用論文

ab64942 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Non-small cell lung cancer line (H1155))
Loading amount 25 µg
Specification Non-small cell lung cancer line (H1155)
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Sep 14 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"