Rabbit polyclonal to Claudin 6
Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human Claudin 6.
This antibody gave a positive signal in the following lysates: A498 (Human Kidney Carcinoma) Whole Cell Lysate
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 23 kDa).
Plays a major role in tight junction-specific obliteration of the intercellular space.
Belongs to the claudin family.
Cell junction > tight junction. Cell membrane.
Information by UniProt
Western blot - Claudin 6 antibody (ab99226)
Anti-Claudin 6 antibody (ab99226) at 1 µg/ml + A498 (Human Kidney Carcinoma) Whole Cell Lysate at 10 µg
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:
Observed band size:
24 kDa (
why is the actual band size different from the predicted?
Additional bands at:
47 kDa. We are unsure as to the identity of these extra bands.
has not yet been referenced specifically in any publications.
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