Rabbit polyclonal to Claudin 6
Synthetic peptide derived from an internal sequence of human Claudin 6.
human ovarian tissue, HUVEC cells, extracts from Jurkat cells.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 23 kDa).
1/50 - 1/100.
1/500 - 1/1000.
Plays a major role in tight junction-specific obliteration of the intercellular space.
Belongs to the claudin family.
Cell junction > tight junction. Cell membrane.
Information by UniProt
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 6 antibody (ab75055)
Human ovarian tissue with ab75055 at 1/50 dilution, in the absence and presence of the immunising peptide.
Immunocytochemistry/ Immunofluorescence - Anti-Claudin 6 antibody (ab75055)
Huvec cells with ab75055 at 1/500 dilution, in the absence and presence of the immunising peptide.
Western blot - Anti-Claudin 6 antibody (ab75055)
All lanes :
Anti-Claudin 6 antibody (ab75055) at 1/500 dilution
Lane 1 :
extracts from Jurkat cells
Lane 2 :
extracts from Jurkat cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size:
Observed band size:
25 kDa (
why is the actual band size different from the predicted?
Additional bands at:
<19 kDa. We are unsure as to the identity of these extra bands.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"