製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Claudin 4
  • アプリケーション
    適用あり: IHC-P, ICC/IF, WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Pig
  • 免疫原

    Synthetic peptide within Human Claudin 4 aa 160-209. The exact sequence is proprietary.
    Database link: O14493

  • ポジティブ・コントロール
    • WB: HeLa cell extracts. IHC-P: Human colon tissue. ICC/IF: MCF7 cells.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS

    Without Mg+2 and Ca+2
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab53156 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
ELISA 1/5000.

ターゲット情報

  • 機能
    Plays a major role in tight junction-specific obliteration of the intercellular space.
  • 関連疾患
    Note=CLDN4 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
  • 配列類似性
    Belongs to the claudin family.
  • 細胞内局在
    Cell junction > tight junction. Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Claudin-4 antibody
    • Claudin4 antibody
    • CLD4_HUMAN antibody
    • CLDN 4 antibody
    • CLDN4 antibody
    • Clostridium perfringens enterotoxin receptor 1 antibody
    • Clostridium perfringens enterotoxin receptor antibody
    • CPE R antibody
    • CPE receptor antibody
    • CPE-R antibody
    • CPE-receptor antibody
    • CPER antibody
    • CPETR 1 antibody
    • CPETR antibody
    • CPETR1 antibody
    • hCPE R antibody
    • WBSCR8 antibody
    • Williams Beuren syndrome chromosome region 8 protein antibody
    • Williams-Beuren syndrome chromosomal region 8 protein antibody
    see all

画像

  • All lanes : Anti-Claudin 4 antibody (ab53156) at 1/500 dilution

    Lane 1 : Untreated HeLa (Human epithelial cell line from cervix adenocarcinoma) cell extracts
    Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell extracts with immunizing peptide


    Predicted band size : 22 kDa
    Observed band size : 22 kDa
  • ab53156 staining Claudin 4 in human colon tissue. Staining is localized to the cell membrane.
    Left panel: ab53156 at 4 µg/ml.

    Right panel: Isotype control.
    Sections were stained using an automated system at room temperature. Sections were rehydrated and antigen retrieved with EDTA pH 9.0. Slides were blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked again for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with hematoxylin and coverslipped. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

  • ICC/IF image of ab53156 staining Claudin 4 (green) in MCF7 (Human breast adenocarcinoma cell line) cells. The cells were fixed in 4% formaldehyde (10 minutes) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53156, 5 µg/ml) overnight at +4°C. The secondary antibody was an Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1 hour. An Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 µM.

参考文献

This product has been referenced in:
  • Pasternak JA  et al. Claudin-4 undergoes age-dependent change in cellular localization on pig jejunal villous epithelial cells, independent of bacterial colonization. Mediators Inflamm 2015:263629 (2015). IHC . Read more (PubMed: 25948883) »
  • Hwang TL  et al. Claudin-4 expression in gastric cancer cells enhances the invasion and is associated with the increased level of matrix metalloproteinase-2 and -9 expression. Oncol Lett 8:1367-1371 (2014). WB ; Human . Read more (PubMed: 25120725) »

See all 4 Publications for this product

レビューと Q&A

Application
Western blot
Sample
Rat Tissue lysate - whole (intestine)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
40 µg
Specification
intestine
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Oct 18 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Ileum)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH 6.0
Permeabilization
No
Specification
Ileum
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Formaldehyde
Username

Alex Pasternak

Verified customer

投稿 Aug 19 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Chimpanzee Tissue sections (Colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9
Permeabilization
No
Specification
Colon
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Feb 12 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (distal ileum)
Loading amount
40 µg
Specification
distal ileum
Gel Running Conditions
Reduced Denaturing (14)
Blocking step
(agent) for 2 hour(s) and 0 minute(s) · Concentration: 30% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Sep 15 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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