The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 22 kDa).
Use at an assay dependent concentration.
1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Plays a major role in tight junction-specific obliteration of the intercellular space.
Note=CLDN4 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
Liu YX et al. Attempt towards a novel classification of triple-negative breast cancer using immunohistochemical markers. Oncol Lett12:1240-1256 (2016).
Read more (PubMed: 27446423) »
Tokuhara Y et al. Claudin-1, but not claudin-4, exhibits differential expression patterns between well- to moderately-differentiated and poorly-differentiated gastric adenocarcinoma. Oncol Lett10:93-98 (2015).
Read more (PubMed: 26170982) »