製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Claudin 3
  • アプリケーション
    適用あり: ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Chicken, Human, Pig
  • 免疫原

    Synthetic peptide corresponding to Mouse Claudin 3 (C terminal).

  • ポジティブ・コントロール
    • Tested with small intestine cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab15102 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/100.
WB Use at an assay dependent concentration. PubMed: 18192323
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
  • 関連疾患
    Note=CLDN3 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
  • 配列類似性
    Belongs to the claudin family.
  • 細胞内局在
    Cell junction > tight junction. Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C7orf1 antibody
    • Claudin-3 antibody
    • Claudin3 antibody
    • CLD3_HUMAN antibody
    • CLDN 3 antibody
    • Cldn3 antibody
    • Clostridium perfringens enterotoxin receptor 2 antibody
    • CPE R2 antibody
    • CPE receptor 2 antibody
    • CPE-R 2 antibody
    • CPE-receptor 2 antibody
    • CPETR 2 antibody
    • CPETR2 antibody
    • HRVP 1 antibody
    • HRVP1 antibody
    • Rat ventral prostate 1 like protein antibody
    • Rat ventral prostate.1 protein homolog antibody
    • RVP1 antibody
    • Ventral prostate.1 like protein antibody
    • Ventral prostate.1 protein homolog antibody
    see all

Anti-Claudin 3 antibody 画像

  • ICC/IF image of ab15102 stained Mcf7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15102, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab15102 (2µg/ml) staining Claudin 3 in human ileum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong membrane staining of the mucosal epithelial cells.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • Immunocytochemistry/ Immunofluorescence analysis of pig primary porcine brain capillary endothelial cells labeling Claudin 3 with ab15102 (undiluted). Cells were fixed with Acetone/Methanol (3:7) and blocked with 10% BSA for 30 minutes at 22°C. A Goat anti-Rabbit IgG (H+L) Alexa Fluor® 488 conjugate secondary antibody was used at 1/200. DAPI nuclear staining.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labeling Claudin 3 with ab15102 at 1/300 dilution. Slides were rehydrated and then boiled for antigen retrieval in TE pH 9 buffer for 15min in standard microwave oven at 650W. After 30min blocking in PBS-Triton 0,1% / BSA 5% slides were incubated overnight with Anti-Claudin 3 antibody (ab15102) at 1/300 dilution at 4°C. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/200 dilution for 1h. Nuclei were stained with Hoechst.

    See Abreview

  • Immunohistochemical analysis of PFA-fixed, paraffin embedded BALB/c murine ear tissue sections.

    Claudin 3 was stained, using ab15102 at 1/10 dilution, in the inner ear at the organ of Corti, Reissner’s membrane, periosteal lining of otic capsule, and the apical surface of the marginal cells of stria vascularis (upper panel) and in the middle ear intercellular junctions along lateral and basal cell membranes in the mucosal epithelium (lower panel). An AlexaFluor®488-conjugated IgG (1/50) was used as the secondary antibody.

Anti-Claudin 3 antibody (ab15102) 使用論文

This product has been referenced in:
  • Kudinov AE  et al. Musashi-2 (MSI2) supports TGF-ß signaling and inhibits claudins to promote non-small cell lung cancer (NSCLC) metastasis. Proc Natl Acad Sci U S A 113:6955-60 (2016). WB, ICC/IF . Read more (PubMed: 27274057) »
  • Krueger M  et al. Blood-brain barrier breakdown involves four distinct stages of vascular damage in various models of experimental focal cerebral ischemia. J Cereb Blood Flow Metab 35:292-303 (2015). Read more (PubMed: 25425076) »

See all 6 Publications for this product

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Mature Porcine Jejunum)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH 6.0
Permeabilization
No
Specification
Mature Porcine Jejunum
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Formaldehyde
Username

Alex Pasternak

Verified customer

投稿 Aug 26 2016

Application
Western blot
Sample
Rat Tissue lysate - whole (Intestinal tissue)
Gel Running Conditions
Reduced Denaturing (4-12% Bis Tris MOPS)
Loading amount
28 µg
Specification
Intestinal tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Sep 07 2015

Application
Western blot
Sample
Human Tissue lysate - whole (Intestinal tissue)
Gel Running Conditions
Reduced Denaturing (4-12% Bis Tris MOPS)
Loading amount
28 µg
Specification
Intestinal tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Aug 04 2015

Application
Western blot
Sample
Mouse Tissue lysate - whole (Intestinal tissue)
Gel Running Conditions
Reduced Denaturing (4-12% Bis Tris MOPS)
Loading amount
28 µg
Specification
Intestinal tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jul 29 2015

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Non-reduced Denaturing (15%)
Sample
Mouse Tissue lysate - whole (lung)
Specification
lung
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Apr 01 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Sample
Chicken Cell (neural fold stage)
Specification
neural fold stage
Permeabilization
Yes - 0.3% tritonX-100 in PBS
Fixative
10% trichloroacetic acid
Username

Ms. Amanda Baumholtz

Verified customer

投稿 Jun 25 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Liver)
Specification
Liver
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TE pH 9.0
Permeabilization
Yes - PBS-Triton 0,1% 10min
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Daniel Alpern

Verified customer

投稿 Aug 06 2012

Thank you for contacting us. We have tested this antibody only in ICC/IF, WB and IHC-Phowevernot inflow cytometry. This antibody can be used in Flow Cytometry however due to lack of tested data we are unable to confirm it. Therefore, I can offer a disc...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Pig Cell (primary porcine brain capillary endothelial cells)
Specification
primary porcine brain capillary endothelial cells
Fixative
Acetone/Methanol (3:7)
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Username

Mrs. Julia Stab

Verified customer

投稿 Oct 14 2011

Thank you for your enquiry and I'm sorry to hear that you are experiencing difficulty with ab15102. This antibody hasn't previously been tested for application in Western blotting, so we don't know if it detects claudin 3 under these conditions. The an...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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