Anti-Claudin 2 抗体 - Aminoterminal end (ab76032)

製品の概要

  • 製品名
    Anti-Claudin 2 antibody - Aminoterminal end
    Claudin 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Claudin 2 - Aminoterminal end
  • アプリケーション
    適用あり: WB, IHC-P, ELISAmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    KLH conjugated synthetic peptide selected from the N terminal region of human Claudin 2

  • ポジティブ・コントロール
    • Mouse heart tissue lysates; Human hepatocarcinoma tissue

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    This antibody is purified through a protein A column, followed by peptide affinity purification.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab76032 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IHC-P
ELISA
  • 追加情報
    ELISA: 1/1000.
    IHC-P: 1/10 - 1/50.
    WB: 1/100 - 1/250. Detects bands of approximately 25 and 50 kDa (predicted molecular weight: 25 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    Anti-Claudin 2 antibody - Aminoterminal end 画像

    • Anti-Claudin 2 antibody - Aminoterminal end (ab76032) at 1/240 dilution + Mouse heart tissue lysates at 35 µg

      Predicted band size : 25 kDa
      Observed band size : 25 kDa
      Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.
    • Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ab76032 at a 1/10 dilution, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

    Anti-Claudin 2 antibody - Aminoterminal end (ab76032) 使用論文

    This product has been referenced in:
    • Miquel S  et al. Anti-nociceptive effect of Faecalibacterium prausnitzii in non-inflammatory IBS-like models. Sci Rep 6:19399 (2016). IHC . Read more (PubMed: 26775847) »
    • Labrie V  et al. Serine racemase is associated with schizophrenia susceptibility in humans and in a mouse model. Hum Mol Genet 18:3227-43 (2009). WB ; Mouse . Read more (PubMed: 19483194) »

    See all 2 Publications for this product

    Product Wall

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated
    Sample
    Rhesus monkey Tissue sections (Colon)
    Specification
    Colon
    Permeabilization
    Yes - 0.1% Triton-X-100
    Fixative
    Z-fix
    Username

    Abcam user community

    Verified customer

    投稿 Oct 22 2014

    Vielen Dank für Ihren Anruf und dafür, dass Sie sich die Zeit genommen haben, unseren Fragebogen auszufüllen.

    Es tut mir leid, dass Sie Probleme mit unserem Claudin 2 Antikörper haben. Da Ihre Bestellung innerhalb der letzt...

    Read More
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Pancreas)
    Loading amount
    20 µg
    Specification
    Pancreas
    Gel Running Conditions
    Reduced Denaturing (10% Bis-Tris from Invitrogen)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5%
    Username

    Joby Westmoreland

    Verified customer

    投稿 Sep 17 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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