製品の概要

  • 製品名Anti-Claudin 2 antibody
    Claudin 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Claudin 2
  • アプリケーション適用あり: ICC/IF, WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide from the C-terminus of Human Claudin 2.

  • ポジティブ・コントロール
    • A431 cell lysate, Skin tissue This antibody gave a positive result when used in the following formaldehyde fixed cell line: HeLa.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーpH: 7.20
    Constituent: 99% PBS
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab125293 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500. Detects a band of approximately 24 kDa (predicted molecular weight: 25 kDa).
IP Use a concentration of 2 - 5 µg/ml.
IHC-P Use a concentration of 1 - 2 µg/ml.

ターゲット情報

Anti-Claudin 2 antibody 画像

  • ICC/IF image of ab125293 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum/ 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab125293 at 5 µg/mL overnight at +4°C. The secondary antibody (green) was DyLight® 488 Goat anti-Rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-Claudin 2 antibody (ab125293) at 1/500 dilution + A431 cell lysate

    Predicted band size : 25 kDa

Anti-Claudin 2 antibody (ab125293) 使用論文

This product has been referenced in:
  • Vanuytsel T  et al. From intestinal permeability to dysmotility: the biobreeding rat as a model for functional gastrointestinal disorders. PLoS One 9:e111132 (2014). WB ; Rat . Read more (PubMed: 25354336) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab125293.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"