製品の概要

  • 製品名Anti-Claudin 14 antibody
    Claudin 14 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to Claudin 14
  • アプリケーション適用あり: ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide:

    SATHSGYRLNDYV

    , corresponding to C terminal amino acids 227-239 of Human Claudin14.

  • ポジティブ・コントロール
    • Human Liver lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab19035 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 26 kDa).
IHC-P Use at an assay dependent dilution. PubMed: 21454667

ターゲット情報

  • 機能Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
  • 組織特異性Liver, kidney. Also found in ear.
  • 関連疾患Defects in CLDN14 are the cause of deafness autosomal recessive type 29 (DFNB29) [MIM:605608]. DFNB29 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
  • 配列類似性Belongs to the claudin family.
  • 細胞内局在Cell junction > tight junction. Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AI851731 antibody
    • Claudin 14 antibody
    • Claudin-14 antibody
    • CLD14_HUMAN antibody
    • CLDN 14 antibody
    • CLDN14 antibody
    • DFNB 29 antibody
    • DFNB29 antibody
    • Human CLDN14 gene antibody
    • MGC109472 antibody
    • OTTHUMP00000109046 antibody
    • OTTHUMP00000109049 antibody
    • OTTMUSP00000021531 antibody
    • UNQ777/PRO1571 antibody
    see all

Anti-Claudin 14 antibody 画像



  • Predicted band size : 26 kDa


    ab19035 (0.3 µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • ab19035 staining Claudin 14 in kidney cells by Immunocytochemistry/ Immunofluorescence.

    Cells were fixed with methanol, permeabilized with Triton X-100 and blocked with 5% BSA for 30 minutes. Samples were incubated with primary antibody (1/600 in PBS + 0.5% BSA + 0.1% Triton X-100) for 1 hour at 20°C. An AlexaFluor®488-conjugated donkey anti-goat polyclonal IgG (1/800) was used as the secondary antibody.

    See Abreview

Anti-Claudin 14 antibody (ab19035) 使用論文

This product has been referenced in:
  • Sato M  et al. Identification of Transcription Factor E3 (TFE3) as a Receptor-independent Activator of G{alpha}16: GENE REGULATION BY NUCLEAR G{alpha} SUBUNIT AND ITS ACTIVATOR. J Biol Chem 286:17766-76 (2011). IHC-P ; Mouse . Read more (PubMed: 21454667) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Non-Denaturing (Native) (15%)
Sample Mouse Tissue lysate - whole (lung)
Specification lung
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

投稿 Mar 16 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (kidney)
Specification kidney
Fixative Methanol
Permeabilization Yes - Triton X
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5%
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Abcam user community

Verified customer

投稿 Sep 05 2012

Thank you for getting back to me. Generally if an immunogen forms an alignment of 6 or more consecutive residues I would consider it possible that the antibody would react against the homolog of the species. However, if it is a polyclonal antisera ...

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Thank you for your enquiry. I have performed a sequence analysis of your fish sequence with all of immunogens used to raise our antibodies against Claudin. Unfortunately none had significant sequence similarity that would make me consider reactivit...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"