製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Claudin 1
  • アプリケーション
    適用あり: IHC-Fr, ICC/IF, WB, IP, IHC-FoFr, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Dog, Monkey
  • 免疫原

    Synthetic peptide (Human) (C terminal).

  • ポジティブ・コントロール
    • Tested with skin and breast carcinoma.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.1% Sodium Azide
    Constituents: 1% BSA, 10mM PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab15098 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.
WB Use at an assay dependent concentration. Predicted molecular weight: 23 kDa.
IP 1/2500.
IHC-FoFr Use at an assay dependent concentration. PubMed: 21719782
IHC-P 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. PubMed: 19284967

ターゲット情報

  • 機能
    Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. CLDN1 is required to prevent the paracellular diffusion of small molecules through tight junctions in the epidermis and is required for the normal barrier function of the skin. Required for normal water homeostasis and to prevent excessive water loss through the skin, probably via an indirect effect on the expression levels of other proteins, since CLDN1 itself seems to be dispensable for water barrier formation in keratinocyte tight junctions (PubMed:23407391).
    (Microbial infection) Acts as a receptor for hepatitis C virus in hepatocytes (PubMed:17325668). Acts as a receptor for dengue virus (PubMed:24074594).
  • 組織特異性
    Strongly expressed in liver and kidney. Expressed in heart, brain, spleen, lung and testis.
  • 関連疾患
    Ichthyosis-sclerosing cholangitis neonatal syndrome
  • 配列類似性
    Belongs to the claudin family.
  • 細胞内局在
    Cell junction, tight junction. Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Claudin-1 antibody
    • Claudin1 antibody
    • CLD 1 antibody
    • CLD1 antibody
    • CLD1_HUMAN antibody
    • CLDN 1 antibody
    • Cldn1 antibody
    • ILVASC antibody
    • SEMP 1 antibody
    • SEMP1 antibody
    • Senescence associated epithelial membrane protein 1 antibody
    • Senescence associated epithelial membrane protein antibody
    • Senescence-associated epithelial membrane protein antibody
    see all

画像

  • ab15098 (1µg/ml) staining Claudin 1 in human skin using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of the cell membrane.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • ICC/IF image of ab15098 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15098, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab15098 staining Claudin 1 in mouse kidney tissue sections by IHC-P (paraformaldehyde-fixed paraffin-embedded sections). Tissue samples were fixed with paraformaldehyde and blocked with 20% serum for 30 minutes at 22°C; antigen retrival was by heat mediation in Citrate buffer (pH6). The sample was incubated with primary antibody (1/500 in blocking buffer) at 22°C for 16 hours. A Biotin-conjugated Donkey polyclonal to rabbit IgG (1/250) was used as secondary antibody.

    See Abreview

  • Human skin stained with ab15098.
  • Anti-Claudin 1 antibody (ab15098) at 1/25 dilution + HeLa cell lysate

    Predicted band size : 23 kDa
    Observed band size : 19 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of acetone-fixed frozen murine mammary gland, labelling Claudin 1 with ab15098 used at a dilution of 1/200 incubated for 16 hours at 4°C in PBS with 0.025% Triton. Blocking was with 10% serum incubated for 1 hour at 25°C. The secondary used was a polyclonal goat anti-rabbit Alexa Fluor® 568 conjugate used at 1/1000.

    See Abreview

参考文献

This product has been referenced in:
  • Liu H  et al. Cycling hypoxia affects cell invasion and proliferation through direct regulation of claudin1 / claudin7 expression, and indirect regulation of P18 through claudin7. Oncotarget 8:10298-10311 (2017). Read more (PubMed: 28055967) »
  • Süren D  et al. Expression of claudin 1, 4 and 7 in thyroid neoplasms. Oncol Lett 13:3722-3726 (2017). Read more (PubMed: 28529587) »

See all 37 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (keratinocytes)
Gel Running Conditions
Reduced Denaturing (15% gel)
Loading amount
30 µg
Treatment
100ng/ml IL-33 for 24hr
Specification
keratinocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Sang Hoon Jeong

Verified customer

投稿 Jun 09 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rhesus monkey Tissue sections (Jejunum)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: High pH for 20 min and then Low pH citrate buffer
Permeabilization
Yes - 0.1% Triton-X-100
Specification
Jejunum
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Z-FIX
Username

Abcam user community

Verified customer

投稿 Oct 10 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Intestinal tissue)
Gel Running Conditions
Reduced Denaturing (4-12% Bis Tris MOPS)
Loading amount
14 µg
Specification
Intestinal tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Sep 06 2016

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (Intestinal tissue)
Gel Running Conditions
Reduced Denaturing (4-12% Bis Tris MOPS)
Loading amount
14 µg
Specification
Intestinal tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Aug 24 2016

Abreviews
Application
Western blot
Sample
Pig Tissue lysate - whole (Intestinal tissue (mucosa))
Gel Running Conditions
Reduced Denaturing (4-12% Bis Tris MOPS)
Loading amount
25 µg
Specification
Intestinal tissue (mucosa)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Aug 17 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (Intestinal tissue)
Gel Running Conditions
Reduced Denaturing (4-12% Bis Tris MOPS)
Loading amount
20 µg
Specification
Intestinal tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Aug 17 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Skin)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Protease type XIV (Sigma P5147)
Permeabilization
No
Specification
Skin
Blocking step
BioGenex Power Block 10 minutes, then BioGenex Protein Block (NGS) 20 minutes as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 24°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Nov 19 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA, pH9, 100C, 20 min
Permeabilization
No
Specification
skin
Blocking step
H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Oct 16 2015

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Mouse Mammary Gland)
Specification
Mouse Mammary Gland
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Acetone
Username

Abcam user community

Verified customer

投稿 Aug 18 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Nerve Bundle adjacent to Temporal Artery Biopsy)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer, pH 6.0
Permeabilization
No
Specification
Nerve Bundle adjacent to Temporal Artery Biopsy
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde
Username

April Rempel

Verified customer

投稿 May 15 2015

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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