製品の概要

  • 製品名Anti-Clathrin antibody
    Clathrin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Clathrin
  • アプリケーション適用あり: ICC/IF, WB, IHC-Fr, IHC-P, IPmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Drosophila melanogaster
    交差が予測される動物種: Chicken, Cow, Xenopus laevis, Caenorhabditis elegans, Zebrafish, a wide range of other species
  • 免疫原

    Synthetic peptide corresponding to Rat Clathrin aa 619-638.
    Sequence:

    KAGLLQRALEHFTDLYDIKR

  • ポジティブ・コントロール
    • Rat lung tissue.

法規制情報

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.02% Thimerosal (merthiolate)
    Constituents: Whole serum
  • 精製度Whole antiserum
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab59710 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/100 - 1/400.
WB 1/1000 - 1/8000. Predicted molecular weight: 192 kDa.
IHC-Fr 1/1000 - 1/4000.
IHC-P Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

ターゲット情報

  • 関連性Membrane vesicle formation is a process required for the endocytosis and biosynthesis of various secreted and membrane bound proteins. Clathrin is a protein which assembles into a polyhedral network on the cell membrane as the membrane invaginates, forming a coated pit which is essential to endocytosis. Clathrin is composed of three polypeptides, a 180 kDa heavy chain and two 32-38 kDa light chains which combine to create a distinct three-legged triskelion. It is this morphology which allows Clathrin to form its unique polyhedral network.
  • 細胞内局在Cytoplasmic face of coated pits and vesicles.
  • 参照データベース
  • 別名
    • Adapter related protein complex 1 beta 1 subunit antibody
    • Adaptor Protein Complex AP1 Beta 1 Subunit antibody
    • ADTB1 antibody
    • AP 1 complex subunit beta 1 antibody
    • AP105A antibody
    • AP1B1 antibody
    • BAM22 antibody
    • Beta Adaptin 1 antibody
    • Beta Prime Adaptin antibody
    • CLAPB2 antibody
    • Clathrin Assembly Protein Complex 1 Beta Large Chain antibody
    • Clathrin heavy chain 1 antibody
    • CLH 17 antibody
    • CLH17 antibody
    • CLTC antibody
    • CLTCL2 antibody
    • Golgi Adaptor HA1/AP1 Adaptin Beta Subunit antibody
    • KIAA0034 antibody
    • Plasma Membrane Adaptor HA2/AP2 Adaptor Beta Subunit antibody
    see all

Anti-Clathrin antibody 画像

  • ab59710 at 1/1000, overnight, secondary antibody biotinylated goat anti-sheep at 1/3000.

    Figure A is a low power image showing intense staining of non-ciliated bronchiolar cells (Clara cells) and lesser staining in alveolar type II cells.

    Figure B is a higher power image of the staining of the non ciliated bronchiolar cells.

  • ab59710 at 1/100, overnight, secondary antibody donkey anti-rabbit Cy2® at 1/50. Confocal image shows intense staining of non-ciliated bronchiolar cells (Clara cells).

Anti-Clathrin antibody (ab59710) 使用論文

This product has been referenced in:
  • Garcin PO & Panté N Cell migration is another player of the minute virus of mice infection. Virology 468-470C:150-159 (2014). Mouse . Read more (PubMed: 25173091) »
  • Fowler R  et al. Uptake and transport of B12-conjugated nanoparticles in airway epithelium. J Control Release 172:374-81 (2013). ICC/IF ; Human . Read more (PubMed: 24008152) »

See all 2 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Sample Fruit fly (Drosophila melanogaster) Cell (Larval neuromuscular junctions (neurons))
Specification Larval neuromuscular junctions (neurons)
Permeabilization Yes - 0.4% Triton-X-100
Fixative Formaldehyde
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投稿 May 13 2014

Application Western blot
Loading amount 100 µg
Gel Running Conditions Reduced Denaturing (3-8% Tris-Acetate Gradient Gel (Invitrogen Novex))
Sample Fruit fly (Drosophila melanogaster) Tissue lysate - whole (whole fly)
Specification whole fly
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
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投稿 May 12 2014

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I am very pleased to hear you would like to accept our offer and test ab59710 in pig. This code will give you xxx off your next order before the...

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Thank you for your call today and for letting us know about the trouble with this antibody. I sincerely apologize that this problem was not initially resolved, and if there is anything else that we can do for you please let me know.

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Nous avons dans notre catalogue des anticorps marqueurs des voies d'internalisation tels que les anti-Clathrin ab2731 (http://www.abcam.com/ab2731) et ab59710 (http://www.abcam.com/ab59710). Malheureusement nous n'avons pas ces anticorps coup...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (Raji)
Total protein in input 10000 cells
Specification Raji
Treatment Goat anti Human IgM F(ab) for 5 minutes
Immuno-precipitation step Protein G
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投稿 May 13 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"