製品の概要

  • 製品名Anti-CLASP2 antibody [KT68]
  • 製品の詳細
    Rat monoclonal [KT68] to CLASP2
  • アプリケーション適用あり: WB, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Fusion protein corresponding to Human CLASP2 (N terminal).

  • ポジティブ・コントロール
    • This antibody gave a positive result in IHC in the following FFPE tissue: Mouse Brain.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab95373 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/5000. Predicted molecular weight: 141 kDa.
ICC/IF 1/100.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex.
  • 組織特異性Brain-specific.
  • 配列類似性Belongs to the CLASP family.
    Contains 5 HEAT repeats.
  • ドメインThe microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends.
  • 翻訳後修飾Phosphorylated by GSK3B. Phosphorylation by GSK3B may negatively regulate binding to microtubule lattices in lamella. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle. Golgi apparatus. Golgi apparatus > trans-Golgi network. Cell membrane. Cell projection > ruffle membrane. Localizes to microtubule plus ends. Localizes to centrosomes, kinetochores and the mitotic spindle from prometaphase. Subsequently localizes to the spindle midzone from anaphase and to the midbody from telophase. In migrating cells localizes to the plus ends of microtubules within the cell body and to the entire microtubule lattice within the lamella. Localizes to the cell cortex and this requires ERC1 and PHLDB2. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CLAP2_HUMAN antibody
    • Clasp2 antibody
    • CLIP associated protein 2 antibody
    • CLIP associating protein 2 antibody
    • CLIP-associating protein 2 antibody
    • Cytoplasmic linker associated protein 2 antibody
    • Cytoplasmic linker-associated protein 2 antibody
    • hOrbit2 antibody
    • KIAA0627 antibody
    • Multiple asters (Mast) like homolog 2 antibody
    • Protein Orbit homolog 2 antibody
    see all

Anti-CLASP2 antibody [KT68] 画像

  • Immunocytochemical immunofluorescence analysis of formaldehyde-fixed SV-40 transformed bovine macrophages, labelling CLASP2 with ab95373 at a dilution of 1/100 incubated for 1 hour at 18°C in 10% FBS. Pereabilization was with 0.2% Triton X-100 for 10 minutes. Blocking used 10% serum incubated for 30 minutes at 18°C. Secondary was a Goat anti-rat polyclonal Alexa Fluor® 488 at 1/1000.

    See Abreview

  • IHC image of CLASP2 staining in Mouse Brain formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab95373, 1 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-CLASP2 antibody [KT68] (ab95373) at 1/1000 dilution

    Lane 1 : Human HEK 293 whole cell lysates
    Lane 2 : BoMAC SV-40 transformed bovine macrophage whole cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    Goat anti-rat polyclonal HRP conjugate at 1/2000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 141 kDa
    Additional bands at : 65 kDa (possible non-specific binding).

    Exposure time : 10 minutes

    Image is courtesy of an AbReview submitted by Kerry Woods.

    From the Left to right: lane 1, lane 2. Blocking was performed with 5% milk incubated for 30 minutes at 18°C.

    See Abreview

Anti-CLASP2 antibody [KT68] (ab95373) 使用論文

ab95373 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Cow Cell (BoMAC (SV40-transformed bovine macrophages))
Permeabilization Yes - 0.2% triton-X.100, 10 min
Specification BoMAC (SV40-transformed bovine macrophages)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 18°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 Oct 26 2015

Application Western blot
Sample Cow Cell lysate - whole cell (BoMAC (SV40-transformed bovine macrophages) and HE)
Gel Running Conditions Reduced Denaturing (10%)
Loading amount 15 µg
Specification BoMAC (SV40-transformed bovine macrophages) and HE
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 18°C
Username

Kerry Woods

Verified customer

投稿 Oct 26 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"