製品の概要

  • 製品名Anti-CLASP1 antibody
    CLASP1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CLASP1
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • 免疫原

    Synthetic peptide corresponding to a region between residues 1488 and 1538 of Human CLASP1 (NP_056097.1)

  • ポジティブ・コントロール
    • HeLa whole cell lysate.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab85919 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 169 kDa.
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 機能Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle.
  • 配列類似性Belongs to the CLASP family.
    Contains 7 HEAT repeats.
  • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle. Golgi apparatus > trans-Golgi network. Localizes to microtubule plus ends. Localizes to centrosomes, kinetochores and the mitotic spindle from prometaphase. Subsequently localizes to the spindle midzone from anaphase and to the midbody from telophase. In migrating cells localizes to the plus ends of microtubules within the cell body and to the entire microtubule lattice within the lamella. Localizes to the cell cortex and this requires ERC1 and PHLDB2.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 1700030C23Rik antibody
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    • CLIP associating protein CLASP1 antibody
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    • Cytoplasmic linker associated protein 1 antibody
    • Cytoplasmic linker-associated protein 1 antibody
    • DKFZp686D1968 antibody
    • DKFZp686H2039 antibody
    • FLJ33821 antibody
    • FLJ41222 antibody
    • hOrbit1 antibody
    • KIAA0622 antibody
    • MAST1 antibody
    • MGC131895 antibody
    • mKIAA0622 antibody
    • Multiple asters 1 antibody
    • Multiple asters homolog 1 antibody
    • Protein Orbit homolog 1 antibody
    see all

Anti-CLASP1 antibody 画像

  • All lanes : Anti-CLASP1 antibody (ab85919) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 169 kDa
    Observed band size : 171 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 115 kDa,52 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • Detection of Human CLASP1 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab85919 at 3 µg/mg lysate for IP and at 0.4 µg/ml for subsequent Western blot detection. Detection: Chemiluminescence with an exposure time of 3 minutes.

Anti-CLASP1 antibody (ab85919) 使用論文

ab85919 has not yet been referenced specifically in any publications.

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