製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to CKMT2
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide from the N terminal region of human CKMT2 conjugated to KLH

  • ポジティブ・コントロール
    • Mouse heart tissue lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab71723 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).

ターゲット情報

  • 機能
    Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
  • 組織特異性
    Sarcomere-specific. Found only in heart and skeletal muscles.
  • 配列類似性
    Belongs to the ATP:guanido phosphotransferase family.
    Contains 1 phosphagen kinase C-terminal domain.
    Contains 1 phosphagen kinase N-terminal domain.
  • 細胞内局在
    Mitochondrion inner membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CKMT 2 antibody
    • Basic-type mitochondrial creatine kinase antibody
    • CKMT2 antibody
    • CPK antibody
    • Creatine kinase mitochondrial 2 antibody
    • Creatine kinase mitochondrial 2 (sarcomeric) antibody
    • Creatine kinase S-type antibody
    • creatine kinase S-type, mitochondrial antibody
    • Creatine kinase, sarcomeric mitochondrial antibody
    • KCRS_HUMAN antibody
    • Mib CK antibody
    • Mib-CK antibody
    • mitochondrial antibody
    • OTTHUMP00000147542 antibody
    • S-MtCK antibody
    • Sarcomeric mitochondrial creatine kinase antibody
    • SMTCK antibody
    see all

画像

  • Anti-CKMT2 antibody (ab71723) at 1/1000 dilution + Human heart tissue lysate at 35 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/5000 dilution

    Predicted band size : 48 kDa
  • All lanes : Anti-CKMT2 antibody (ab71723) at 1/1000 dilution

    Lane 1 : Human skeletal muscle tissue lysate
    Lane 2 : Human heart tissue lysate

    Lysates/proteins at 35 µg per lane.

    Secondary
    HRP goat anti-rabbit (H+L) at 1/5000 dilution

    Predicted band size : 48 kDa
  • Anti-CKMT2 antibody (ab71723) at 1/100 dilution + mouse heart tissue lysate at 12.5 µg

    Predicted band size : 48 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

ab71723 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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