製品の概要

  • 製品名Anti-CKMT2 antibody
    CKMT2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CKMT2
  • アプリケーション適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Mouse, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • 免疫原

    A region within synthetic peptide: VCAEVREQPR LFPPSADYPD LRKHNNCMAE CLTPAIYAKL RNKVTPNGYT, corresponding to N terminal amino acids 37-86 of Human CKMT2

  • ポジティブ・コントロール
    • Jurkat cell lysate Human skeletal muscle tissue

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab55963 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 48 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/312500.

Titre using peptide based assay 1/312500.

IHC-P Use a concentration of 4 - 8 µg/ml.

ターゲット情報

Anti-CKMT2 antibody 画像

  • Anti-CKMT2 antibody (ab55963) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 48 kDa
    Observed band size : 46 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffine-embedded sections) analysis of human muscle tissue labelling CKMT2 with ab55963 at 4-8µg/ml. Arrows indicate positively labelled skeletal muscle cells. Magnification: 400X.

Anti-CKMT2 antibody (ab55963) 使用論文

This product has been referenced in:
  • Martin SA  et al. Parallel high-throughput RNA interference screens identify PINK1 as a potential therapeutic target for the treatment of DNA mismatch repair-deficient cancers. Cancer Res 71:1836-48 (2011). WB ; Human . Read more (PubMed: 21242281) »
  • Bansal A  et al. Proteomic analysis reveals late exercise effects on cardiac remodeling following myocardial infarction. J Proteomics 73:2041-9 (2010). WB ; Rat . Read more (PubMed: 20601275) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (brown adipose tissue)
Gel Running Conditions Reduced Denaturing (10% gel)
Loading amount 8 µg
Treatment by a drug which is unknown whether it stimulates or inhibits CKMT2
Specification brown adipose tissue
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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投稿 Apr 29 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"