Anti-CKII alpha prime polypeptide 抗体 (ab10474)

製品の概要

  • 製品名
    Anti-CKII alpha prime polypeptide antibody
  • 製品の詳細
    Rabbit polyclonal to CKII alpha prime polypeptide
  • 特異性
    Reacts with the alpha prime subunit.
  • アプリケーション
    適用あり: IP, ICC/IF, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rabbit, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat, Elephant
  • 免疫原

    Immunogen was a synthetic peptide, which represented a portion of human Casein kinase 2, alpha 2 polypeptide encoded within exon 11 (LocusLink ID 1459).

  • ポジティブ・コントロール
    • RIPA extract from HeLa cells.
  • 特記事項


    CKII is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The gene for this protein is found on chromosome 20, but a related transcribed pseudogene is found on chromosome 11. Three transcript variants have been found, encoding two different isoforms. Protein kinase CKII is recognized as one of the key cellular signals for cell growth and proliferation; it also has an important role in apoptosis.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.1% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Antibodies were affinity purified using the peptide immobilized on solid support.
  • 一次抗体 備考
    CKII is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The gene for this protein is found on chromosome 20, but a related transcribed pseudogene is found on chromosome 11. Three transcript variants have been found, encoding two different isoforms. Protein kinase CKII is recognized as one of the key cellular signals for cell growth and proliferation; it also has an important role in apoptosis.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab10474 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at 4-10 µg/mg of lysate.
ICC/IF Use at an assay dependent concentration.
WB 1/1000 - 1/5000. Detects a band of approximately 39 kDa (predicted molecular weight: 41 kDa).

ターゲット情報

  • 機能
    Catalytic subunit of a constitutively active serine/threonine-protein kinase complex that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine. Regulates numerous cellular processes, such as cell cycle progression, apoptosis and transcription, as well as viral infection. May act as a regulatory node which integrates and coordinates numerous signals leading to an appropriate cellular response. During mitosis, functions as a component of the p53/TP53-dependent spindle assembly checkpoint (SAC) that maintains cyclin-B-CDK1 activity and G2 arrest in response to spindle damage. Also required for p53/TP53-mediated apoptosis, phosphorylating 'Ser-392' of p53/TP53 following UV irradiation. Can also negatively regulate apoptosis. Phosphorylates the caspases CASP9 and CASP2 and the apoptotic regulator NOL3. Phosphorylation protects CASP9 from cleavage and activation by CASP8, and inhibits the dimerization of CASP2 and activation of CASP8. Regulates transcription by direct phosphorylation of RNA polymerases I, II, III and IV. Also phosphorylates and regulates numerous transcription factors including NF-kappa-B, STAT1, CREB1, IRF1, IRF2, ATF1, SRF, MAX, JUN, FOS, MYC and MYB. Phosphorylates Hsp90 and its co-chaperones FKBP4 and CDC37, which is essential for chaperone function. Regulates Wnt signaling by phosphorylating CTNNB1 and the transcription factor LEF1. Acts as an ectokinase that phosphorylates several extracellular proteins. During viral infection, phosphorylates various proteins involved in the viral life cycles of EBV, HSV, HBV, HCV, HIV, CMV and HPV.
  • 配列類似性
    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Casein kinase 2 alpha prime polypeptide antibody
    • Casein kinase II alpha' chain antibody
    • Casein kinase II subunit alpha' antibody
    • CK II alpha' antibody
    • CK II antibody
    • CK2A2 antibody
    • CKII antibody
    • CSK22_HUMAN antibody
    • CSNK2A1 antibody
    • CSNK2A2 antibody
    • FLJ43934 antibody
    see all

画像



  • Predicted band size : 41 kDa


    Detection of human CKII alpha prime polypeptide by Western blot and immunoprecipitation. A. RIPA extract (30 mg) from HeLa cells treated with CKII alpha siRNA or vimentin siRNA (v). B. RIPA extract (30 mg) from HeLa cells treated with CKII alpha, CKII alpha prime, or vimentin (v) siRNA. C. RIPA extract (0.5mg) from HeLa cells. Antibody: ab10474 used at 0.33 (A) or 0.2 (B) mg/ml for WB or 5 ug/0.5 mg lysate (C) for IP.
  • All lanes : Anti-CKII alpha prime polypeptide antibody (ab10474) at 1/500 dilution

    Lane 1 : Whole cell lysate prepared from alpha prime knock out MEFs
    Lane 2 : Whole cell lysate prepared from alpha prime heterozygous MEFs

    Lysates/proteins at 40 µg per lane.

    Secondary
    Goat anti rabbit IgG-HRP at 1/5000 dilution
    Developed using the ECL technique

    Predicted band size : 41 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 minutes

    Image courtesy of an anonymous Abreview.

    See Abreview

参考文献

This product has been referenced in:
  • Tsuchiya Y  et al. The casein kinase 2-nrf1 axis controls the clearance of ubiquitinated proteins by regulating proteasome gene expression. Mol Cell Biol 33:3461-72 (2013). Read more (PubMed: 23816881) »
  • McFarland TP  et al. The cytosolic protein kinase CK2 phosphorylates cardiac calsequestrin in intact cells. Mol Cell Biochem 353:81-91 (2011). WB ; Human . Read more (PubMed: 21431367) »

See all 3 Publications for this product

レビューと Q&A

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Sample
Human Cell (Human primary fibroblasts)
Specification
Human primary fibroblasts
Permeabilization
Yes - 0.2% Triton-X100
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Dec 12 2013

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse T-cell lymphoma and splenocytes)
Loading amount
40 µg
Specification
Mouse T-cell lymphoma and splenocytes
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Tomar Ghansah

Verified customer

投稿 Aug 21 2012

Thank you for your reply.

We only have the full size vials of all of our products, so unfortunatelywe don't have a small sample to send out.However, we will guarantee that this antibody is specific for CKII alpha prime and will work in the te...

Read More

Thank you for your call today and for your patience while I have been in touch with the lab about ab10474.

The immunogen of ab10474 was taken from a region of divergence between CKII alpha and CKII alpha', so the antibody is not cross-reactiv...

Read More
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)
Loading amount
20 µg
Specification
Mouse embryonic fibroblasts
Gel Running Conditions
Reduced Denaturing (10% Tris-Gly gel)
Blocking step
LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Nov 23 2011

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse Embryonic Fibroblasts (MEFs))
Loading amount
40 µg
Specification
Mouse Embryonic Fibroblasts (MEFs)
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Jul 20 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HeLa cells)
Total protein in input
200 µg
Specification
HeLa cells
Immuno-precipitation step
Protein A/G
Username

Abcam user community

Verified customer

投稿 Jan 18 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Loading amount
30 µg
Specification
HeLa cells
Treatment
siRNA to CKII alpha prime
Gel Running Conditions
Reduced Denaturing (10% Tris-Gly)
Blocking step
LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
Username

Dr. Svetlana Khoronenkova

Verified customer

投稿 Jul 22 2010

Thank you for your enquiry and patience. Under the conditions tested, which included WB detection at optimized dilutions using lysate from cells expressing endogenous amounts of the subunits, they were specific as specified. That is, ab10466, ab10468 a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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