Anti-CSNK2A2 抗体 (ab10474)
Key features and details
- Rabbit polyclonal to CSNK2A2
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-CSNK2A2 antibody
CSNK2A2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CSNK2A2 -
由来種
Rabbit -
特異性
Reacts with the alpha prime subunit. -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rabbit, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat, Elephant -
免疫原
Synthetic peptide corresponding to Human CSNK2A2 aa 300 to the C-terminus.
Database link: 1459 -
ポジティブ・コントロール
- WB: HeLa, HEK293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates. IP: HeLa whole cell lysate.
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特記事項
CKII is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The gene for this protein is found on chromosome 20, but a related transcribed pseudogene is found on chromosome 11. Three transcript variants have been found, encoding two different isoforms. Protein kinase CKII is recognized as one of the key cellular signals for cell growth and proliferation; it also has an important role in apoptosis.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Antibodies were affinity purified using the peptide immobilized on solid support. -
一次抗体 備考
CKII is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The gene for this protein is found on chromosome 20, but a related transcribed pseudogene is found on chromosome 11. Three transcript variants have been found, encoding two different isoforms. Protein kinase CKII is recognized as one of the key cellular signals for cell growth and proliferation; it also has an important role in apoptosis. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab10474の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (4) |
1/1000 - 1/5000. Detects a band of approximately 39 kDa (predicted molecular weight: 41 kDa).
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IP | (1) |
Use at 2-10 µg/mg of lysate.
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特記事項 |
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WB
1/1000 - 1/5000. Detects a band of approximately 39 kDa (predicted molecular weight: 41 kDa). |
IP
Use at 2-10 µg/mg of lysate. |
ターゲット情報
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機能
Catalytic subunit of a constitutively active serine/threonine-protein kinase complex that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine. Regulates numerous cellular processes, such as cell cycle progression, apoptosis and transcription, as well as viral infection. May act as a regulatory node which integrates and coordinates numerous signals leading to an appropriate cellular response. During mitosis, functions as a component of the p53/TP53-dependent spindle assembly checkpoint (SAC) that maintains cyclin-B-CDK1 activity and G2 arrest in response to spindle damage. Also required for p53/TP53-mediated apoptosis, phosphorylating 'Ser-392' of p53/TP53 following UV irradiation. Can also negatively regulate apoptosis. Phosphorylates the caspases CASP9 and CASP2 and the apoptotic regulator NOL3. Phosphorylation protects CASP9 from cleavage and activation by CASP8, and inhibits the dimerization of CASP2 and activation of CASP8. Regulates transcription by direct phosphorylation of RNA polymerases I, II, III and IV. Also phosphorylates and regulates numerous transcription factors including NF-kappa-B, STAT1, CREB1, IRF1, IRF2, ATF1, SRF, MAX, JUN, FOS, MYC and MYB. Phosphorylates Hsp90 and its co-chaperones FKBP4 and CDC37, which is essential for chaperone function. Regulates Wnt signaling by phosphorylating CTNNB1 and the transcription factor LEF1. Acts as an ectokinase that phosphorylates several extracellular proteins. During viral infection, phosphorylates various proteins involved in the viral life cycles of EBV, HSV, HBV, HCV, HIV, CMV and HPV. -
配列類似性
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily.
Contains 1 protein kinase domain. - Information by UniProt
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参照データベース
- Entrez Gene: 282420 Cow
- Entrez Gene: 1459 Human
- Entrez Gene: 13000 Mouse
- Omim: 115442 Human
- SwissProt: P20427 Cow
- SwissProt: P19784 Human
- SwissProt: O54833 Mouse
- Unigene: 82201 Human
see all -
別名
- Casein kinase 2 alpha prime polypeptide antibody
- Casein kinase II alpha' chain antibody
- Casein kinase II subunit alpha' antibody
see all
画像
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Detection of human and mouse CSNK2A1 by western blot.
Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer.
Antibody: Affinity purified rabbit anti-CSNK2A1 antibody A300-199A (lot A300-199A-5) used for WB at 0.1 µg/ml.
Detection: Chemiluminescence -
CSNK2A2 was immunoprecipitated from 1mg of HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate with ab10474 at 6 µg/ml. Western blot was performed from the immunoprecipitate using ab10474 at 1 µg/ml.
Lane 1: ab10474 IP in HeLa whole cell lysate.
Lane 2: Ctrl IgG instead of ab10474.
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Detection of human CSNK2A2 by Western blot and immunoprecipitation.
A. RIPA extract (30 mg) from HeLa cells treated with CSNK2A2 siRNA or vimentin siRNA (v).
B. RIPA extract (30 mg) from HeLa cells treated with CSNK2A2, CSNK2A2 prime, or vimentin (v) siRNA.
C. RIPA extract (0.5mg) from HeLa cells.
Antibody: ab10474 used at 0.33 (A) or 0.2 (B) mg/ml for WB or 5 mg/0.5 mg lysate (C) for IP.
Detection of human CSNK2A2 by Western blot and immunoprecipitation. A. RIPA extract (30 mg) from HeLa cells treated with CSNK2A2 siRNA or vimentin siRNA (v). B. RIPA extract (30 mg) from HeLa cells treated with CSNK2A2, CSNK2A2, or vimentin (v) siRNA. C. RIPA extract (0.5mg) from HeLa cells. Antibody: ab10474 used at 0.33 (A) or 0.2 (B) mg/ml for WB or 5 ug/0.5 mg lysate (C) for IP.
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All lanes : Anti-CSNK2A2 antibody (ab10474) at 1/500 dilution
Lane 1 : Whole cell lysate prepared from alpha prime knock out MEFs
Lane 2 : Whole cell lysate prepared from alpha prime heterozygous MEFs
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat anti rabbit IgG-HRP at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 41 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Exposure time: 30 minutes
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab10474 は 5 報の論文で使用されています。
- Beale AD et al. Casein Kinase 1 Underlies Temperature Compensation of Circadian Rhythms in Human Red Blood Cells. J Biol Rhythms 34:144-153 (2019). PubMed: 30898060
- Hoque A et al. Mitochondrial fission protein Drp1 inhibition promotes cardiac mesodermal differentiation of human pluripotent stem cells. Cell Death Discov 4:39 (2018). WB . PubMed: 29531836
- Tsuchiya Y et al. The casein kinase 2-nrf1 axis controls the clearance of ubiquitinated proteins by regulating proteasome gene expression. Mol Cell Biol 33:3461-72 (2013). PubMed: 23816881
- McFarland TP et al. The cytosolic protein kinase CK2 phosphorylates cardiac calsequestrin in intact cells. Mol Cell Biochem 353:81-91 (2011). WB ; Human . PubMed: 21431367
- Jacks KA & Koch CA Differential regulation of mitogen- and stress-activated protein kinase-1 and -2 (MSK1 and MSK2) by CK2 following UV radiation. J Biol Chem 285:1661-70 (2010). WB . PubMed: 19933278