Anti-CKII alpha (phospho T360 + S362) 抗体 (ab119410)

製品の概要

  • 製品名Anti-CKII alpha (phospho T360 + S362) antibody
    CKII alpha 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CKII alpha (phospho T360 + S362)
  • 特異性ab119410 detects endogenous levels of Human CKII alpha only when phosphorylated at Threm=onine 360/Srine 362
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide conjugated to KLH, surrounding amino acids (VTPpPSpPL) phosphorylated at threonine 360 and Serine 362 of Human CKII alpha (NP_001894.2).

  • ポジティブ・コントロール
    • Recombinant proteins CKII alpha wt, CKII alpha T360A, CKII alpha S362A, CKII alphaT360/S362A phoshorylated by ERK2

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
    Note: (without Mg2+ and Ca2+)
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab119410 was purified by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab119410 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 45 kDa).

ターゲット情報

  • 機能Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha and alpha' chains contain the catalytic site. Participates in Wnt signaling. CK2 phosphorylates 'Ser-392' of p53/TP53 following UV irradiation.
  • 配列類似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Casein kinase 2 alpha 1 polypeptide antibody
    • Casein kinase II alpha 1 antibody
    • Casein kinase II alpha 1 subunit antibody
    • Casein kinase II alpha subunit antibody
    • Casein kinase II subunit alpha antibody
    • CK II alpha antibody
    • CK II antibody
    • CK2 alpha antibody
    • CK2 catalytic subunit alpha antibody
    • CK2A1 antibody
    • CKII antibody
    • CKIIalpha antibody
    • CSK21_HUMAN antibody
    • CSNK2A1 antibody
    see all

Anti-CKII alpha (phospho T360 + S362) antibody 画像

  • All lanes : Anti-CKII alpha (phospho T360 + S362) antibody (ab119410) at 1/1000 dilution

    Lane 1 : CKII alpha
    Lane 2 : CKII alpha + ERK2
    Lane 3 : CKII alpha T360A
    Lane 4 : CKII alpha T360A + ERK2
    Lane 5 : CKII alpha S362A
    Lane 6 : CKII alpha S362A + ERK2
    Lane 7 : CKII alpha T360/S362A
    Lane 8 : CKII alpha T360/S362A + ERK2


    Predicted band size : 45 kDa
  • Anti-CKII alpha (phospho T360 + S362) antibody (ab119410) at 1/1000 dilution + C57BL/6 Mouse splenocyte whole cell lysate at 40 µg

    Secondary
    HRP-conjugated goat anti-rabbit monoclonal IgG at 1/1000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 45 kDa
    Observed band size : 45 kDa


    Exposure time : 1 minute

    This image is courtesy of an Abreview submitted by Dr Tomar Ghansah

    See Abreview

Anti-CKII alpha (phospho T360 + S362) antibody (ab119410) 使用論文

ab119410 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (C57BL/6 Mouse splenocytes)
Loading amount 40 µg
Specification C57BL/6 Mouse splenocytes
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Tomar Ghansah

Verified customer

投稿 Oct 05 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"