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Immunogen was a synthetic peptide representing a portion of human Casein kinase 2, alpha 1 polypeptide which lies somewhere between aa300 and aa350.
Our Abpromise guarantee covers the use of ab10468 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/3000 - 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 45 kDa).|
|IHC-P||1/250 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 1 - 5 µg/ml.|
Western blot analysis of RIPA extract (30 µg) from HeLa cells treated with CKII alpha siRNA or vimentin siRNA (v). ab10468 was used at 0.33 or 0.1 µg/ml.
ab10468 has not yet been referenced specifically in any publications.