製品の概要

  • 製品名Anti-CKAP4 antibody
    CKAP4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CKAP4
  • アプリケーション適用あり: ICC/IF, IHC-P, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 216-265 (SDGIHVVKDA RERDFTSLEN TVEERLTELT KSINDNIAIF TEVQKRSQKE) of Human CKAP4 (NP_006816).

  • ポジティブ・コントロール
    • HeLa cell lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab84712 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 4 - 8 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 66 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

Anti-CKAP4 antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bronchial epithelial tissue labelling CKAP4 with ab84712 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the membrane and cytoplasm. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - CKAP4. Right - Merge.
  • Anti-CKAP4 antibody (ab84712) at 1 µg/ml (in 5% skim milk / PBS buffer) + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 66 kDa
    Observed band size : 64 kDa (why is the actual band size different from the predicted?)
    Gel concentration: 12%
  • ICC/IF image of ab84712 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84712, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue labelling CKAP4 with ab84712 at 4-8µg/ml.

Anti-CKAP4 antibody (ab84712) 使用論文

This product has been referenced in:
  • Ortega A  et al. Endoplasmic reticulum stress induces different molecular structural alterations in human dilated and ischemic cardiomyopathy. PLoS One 9:e107635 (2014). WB ; Human . Read more (PubMed: 25226522) »
  • Clark R  et al. Comparative Genomic and Proteomic Analysis of Cytoskeletal Changes inDexamethasone-Treated Trabecular Meshwork Cells. Mol Cell Proteomics : (2012). WB ; Human . Read more (PubMed: 23105009) »

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain)
Permeabilization Yes - 0.3% Troton X-100
Specification Brain
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
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投稿 Oct 27 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Monkey Cell (Liver)
Permeabilization Yes - 0.1% Troton X-100
Specification Liver
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
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投稿 Oct 10 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (neuroblastoma)
Permeabilization Yes - 0.1% Troton X-100
Specification neuroblastoma
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
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投稿 Oct 10 2016

Application Western blot
Sample Mouse Cell lysate - whole cell (neuroblastoma)
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Loading amount 30 µg
Specification neuroblastoma
Blocking step BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
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投稿 Aug 10 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Kidney)
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Loading amount 30 µg
Specification Kidney
Blocking step BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
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投稿 Aug 10 2016

Application Western blot
Sample African Green Monkey Cell lysate - whole cell (Kidney)
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Loading amount 30 µg
Specification Kidney
Blocking step BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
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投稿 Aug 10 2016

Application Western blot
Sample Cow Cell lysate - whole cell (Lung)
Gel Running Conditions Non-reduced Non-Denaturing (Native) (4-12)
Loading amount 30 µg
Specification Lung
Blocking step BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
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投稿 Aug 10 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"