製品の概要

  • 製品名Anti-CKAP2 antibody
    CKAP2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CKAP2
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Gorilla, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region between residues 633 and 683 of human CKAP2 (NP_001091995.1).

  • ポジティブ・コントロール
    • HeLa whole cell lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab85889 was affinity purified using an epitope specific to CKAP2 immobilized on solid support.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab85889 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 77 kDa.
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 機能Possesses microtubule stabilizing properties. Involved in regulating aneuploidy, cell cycling, and cell death in a p53/TP53-dependent manner.
  • 組織特異性Abundant in testis, thymus, and in tumor derived cell lines, while barely detectable in liver, prostate, and kidney.
  • 配列類似性Belongs to the CKAP2 family.
  • 発生段階Present at the G1/S boundary. Accumulates as cells progress from S to G2 into mitosis. Rapidly degraded during mitosis exit by CDH1-activated anaphase promoting complex/cyclosome (APC/C).
  • 細胞内局在Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. Contrary to the ectopically expressed protein, endogenous CKAP2 does not colocalize with microtubules in G1, S and early G2. At late G2 and prophase after separation of duplicated centrosomes, colocalizes with gamma-tubulin and centrosome-proximal microtubules. From prometaphase through anaphase B, colocalizes with mitotic spindle poles and spindle microtubules. During cytokinesis, absent from midbody microtubules.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AI461656 antibody
    • AW228814 antibody
    • CKAP 2 antibody
    • Ckap2 antibody
    • CKAP2_HUMAN antibody
    • CTCL tumor antigen se20 10 antibody
    • CTCL tumor antigen se20-10 antibody
    • Cytoskeleton associated protein 2 antibody
    • Cytoskeleton-associated protein 2 antibody
    • DKFZp686L1238 antibody
    • FLJ10749 antibody
    • LB1 antibody
    • se20 10 antibody
    • TMAP antibody
    • Tumor and microtubule associated protein antibody
    • Tumor associated microtubule associated protein antibody
    • Tumor- and microtubule-associated protein antibody
    see all

Anti-CKAP2 antibody 画像

  • All lanes : Anti-CKAP2 antibody (ab85889) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 77 kDa
    Observed band size : 77 kDa


    Exposure time : 90 seconds
  • Detection of CKAP2 by Western Blot of Immunprecipitate.
    ab85889, at 1 µg/ml, staining CKAP2 in HeLa whole cell lysate immunoprecipitated using ab85889 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 90 seconds.

Anti-CKAP2 antibody (ab85889) 使用論文

ab85889 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab85889.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"