製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to CK1 epsilon
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Dog
  • 免疫原

    Synthetic peptide:

    PASQTSVPFDHLGK

    (human) from the C terminus of the CK1 epsilon protein sequence according to NP_001885.1; NP_689407.1.

  • ポジティブ・コントロール
    • Transfected HEK293 transiently expressing CK1 epsilon.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab77336 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 48 kDa).

ターゲット情報

  • 機能
    Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. Can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates DVL1. Central component of the circadian clock. May act as a negative regulator of circadian rhythmicity by phosphorylating PER1 and PER2. Retains PER1 in the cytoplasm. Inhibits cytokine-induced granuloytic differentiation.
  • 組織特異性
    Expressed in all tissues examined, including brain, heart, lung, liver, pancreas, kidney, placenta and skeletal muscle. Expressed in monocytes and lymphocytes but not in granulocytes.
  • 配列類似性
    Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
    Contains 1 protein kinase domain.
  • 翻訳後修飾
    Autophosphorylated.
  • 細胞内局在
    Cytoplasm. Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Casein kinase 1 epsilon antibody
    • Casein kinase I antibody
    • Casein kinase I epsilon antibody
    • Casein kinase I isoform epsilon antibody
    • CKI epsilon antibody
    • CKI-epsilon antibody
    • CKIe antibody
    • CKIepsilon antibody
    • CSNK 1E antibody
    • CSNK1E antibody
    • DBT antibody
    • Doubletime, Drosophila, homolog of antibody
    • EC 2.7.11.1 antibody
    • epsilon isoform antibody
    • HCK IE antibody
    • KC1E antibody
    • KC1E_HUMAN antibody
    • kinase CK1 epsilon antibody
    • MGC 10398 antibody
    see all

画像

  • Anti-CK1 epsilon antibody (ab77336) at 1 µg/ml + HEK293 cell lysate in RIPA buffer at 35 µg

    Predicted band size : 48 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-CK1 epsilon antibody (ab77336) at 0.3 µg/ml

    Lane 1 : Cell lysates prepared from mock transfected HEK293 cells.
    Lane 2 : Cell lysates prepared from CK1 epsilon transfected HEK293 cells.


    Predicted band size : 48 kDa

プロトコール

参考文献

ab77336 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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